用基因表現圖譜分辨傳統中醫的四逆湯證

The Classification of Sini Decoction Pattern in Traditional Chinese Medicine by Gene Expression Profiling

鄭宏足(Cheng, Hung Tsu)

指導教授 ： 許志楧

國立清華大學/工學院/奈米工程與微系統研究所/博士(2016年)

摘要

｢四逆湯證｣是傳統中醫在臨床上常被診斷出來的證型，我們依據《傷寒雜病論》 的描述來設計問卷，請中醫師們協助評分，得到兩組症狀：｢脈沉微欲絕｣與｢四肢厥冷｣，作為專家共識的四逆湯證診斷標準。接著，以此診斷標準收集了 24 位患者的血液樣本，進行兩個迴圈 48 片的基因微陣列晶片雜合實驗。其中 6 個四逆湯證及 6 個非四逆湯證的樣本作為｢迴圈一」訓練組，用訓練組的基因表現圖譜，來尋找四逆湯證的生物標記基因。另外的 6 個四逆湯證及 6 個非四逆湯證的樣本則作為｢迴圈二｣測試組，在測試組中，將這些生物標記基因的表現圖譜進行相同條件的階層分群，可以成功的將測試組的 12 個樣本區分出四逆湯證群與非四逆湯證群。研究成果顯示，生物標記基因的表現圖譜，可以強化傳統中醫證型的科學化診斷，提供傳統中醫在現代分子生物學領域裡新的風貌與詮釋。

關鍵字

四逆湯證 ； 傳統中醫 ； 證 ； 基因表現圖譜 ； 基因微陣列晶片 ； 生物標記基因

並列摘要

We investigated the syndromes of the Sini decoction pattern (SDP). To obtain a
common profile for SDP, we distributed questionnaires to 300 clinical traditional Chinese
medicine practitioners. According to the survey, we concluded 2 sets of symptoms for SDP:
(1) pulse feels deep or faint and (2) reversal cold of the extremities. Then we collected 24 individuals, and extracted their total mRNA of peripheral blood mononuclear cells for cDNA microarray experiments. 12 samples were used as the training set to identify the biomarkers for distinguishing the SDP and non-SDP groups. The remaining 12 samples were used as the test set. The test results indicated that the gene expression profiles of the
identified biomarkers could effectively distinguish the 2 groups.

並列關鍵字

Sini decoction pattern (SDP) ； traditional Chinese medicine (TCM) ； ZHENG ； cDNA microarray ； gene expression profiling ； biomarkers

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