The Study of a Diacylglycerol Lipase Gene Expression on the modulation of Caenorhabditis elegans Lifespan

二醯甘油酯脂解酶基因表現對線蟲壽命調控之研究

高慈佑(Tzu-Yu Kao)

指導教授 ： 汪宏達

國立清華大學/生命科學院/生物科技研究所/碩士(2009年)

摘要

Genetically prolonged lifespan has been reported to be associated with better capability to resist to environmental stresses. In the previous fly work, we found a long-lived mutant with up-regulation of diacylglycerol lipase (DAGL) gene, inaE, and the DAGL transgenic fly displayed enhanced lifespan. Yet the role of DAGL in C. elegans lifespan remains unknown. Here, I demonstrated that the transgenic worm overexpressing F42G9.6, the DAGL orthologous gene, increases lifespan and RNA-interfered expression of DAGL decreases lifespan in C. elegans. On the other hand, the DAGL mutants exhibit decreased lifespan and lowered resistance to oxidative stress. The longevity and stress resistance of daf-2 partially depends on the function of DAGL and DAGL may be a target of DAF-16 which regulates the transcript expression level of DAGL. The knocking-down either DAG kinase or oligopeptide-transporter-2, which both genes are involved in TOR pathway, rescue the short-lived defect of the DAGL mutants. In summary, we propose that F42G9.6 is likely to catalyze the hydrolysis of DAG and overexpression of F42G9.6 plays an important function in TOR signaling to retard aging of C. elegans.

關鍵字

壽命 ； 線蟲 ； 酯解酶

並列摘要

無資料

並列關鍵字

lifespan ； C. elegans ； lipase

參考文獻

Abraham, R.T. (2002). Identification of TOR signaling complexes: more TORC for the cell growth engine. Cell 111, 9-12.

Avruch, J., Belham, C., Weng, Q., Hara, K., and Yonezawa, K. (2001). The p70 S6 kinase integrates nutrient and growth signals to control translational capacity. Prog Mol Subcell Biol 26, 115-154.

Bluher, M., Kahn, B.B., and Kahn, C.R. (2003). Extended longevity in mice lacking the insulin receptor in adipose tissue. Science 299, 572-574.

Carter, C.S., Ramsey, M.M., and Sonntag, W.E. (2002). A critical analysis of the role of growth hormone and IGF-1 in aging and lifespan. Trends Genet 18, 295-301.

Chyb, S., Raghu, P., and Hardie, R.C. (1999). Polyunsaturated fatty acids activate the Drosophila light-sensitive channels TRP and TRPL. Nature 397, 255-259.