表面電漿磁光柯爾增強效應用於生物感測晶片

Strong Transverse Magneto-optical Kerr Effect on Surface Plasmonic Grating for Sensitive and Label-free Sensing

周桂勗(Kuei-Hsu Chou)

指導教授 ： 李明昌

國立清華大學/電機資訊學院/光電工程研究所/碩士(2013年)

https://doi.org/10.6843/NTHU.2013.00002

摘要

在本論文中，我們主要研究橫向磁光柯爾效應(TMOKE)在光柵式
Au/Fe/Au 結構的表面電漿增強效應用在非標定、高敏感度的光學生
物量測。藉由材料選擇與結構優化來達到最佳化磁光訊號，之後整合
微流道封裝成面積25´14mm2的元件來做流體量測。我們分析了元件的
磁光特性與生物量測的能力。所量測到的最大磁光訊號約為0.035 並
能觀察到磁光訊號在表面電漿共振波長附近具有相當大的色散特性。
接著透過整合好的磁光感測晶片來量測不同濃度的食鹽水，所量測的
本質解析度與食鹽水(bulk solution)解析度分別約為 10-7 RIU
∼、0.004%(wt)。此外，我們利用高親和力的avidin/bBSA 生物組合動態
(real time)量測avidin 與biotin 之間的交互作用，並驗證表面電漿磁光
訊號具有線性量測與定量分析的特性。量測avidin 的解析度約為
1.5nM。最後，我們所設計的感測晶片可由奈米技術生產並整合微流
道成晶片實驗室(lab-on-chip)作為生物感測器。

關鍵字

磁光柯爾效應 ； 表面電漿 ； 感測器

並列摘要

In this thesis, a novel transverse magneto-optical Kerr effect (TMOKE) on the
composite surface plasmon grating is proposed and developed to implement a
label-free, high-sensitive optical biosensor. The Au/Fe/Au grating structure is
designed and optimized to achieve maximal Kerr parameter. The device area is 25x14
mm2 and is integrated with a single microfludic channel for delivering liquid for test.
After fabricating the device, we characterize the magneto-optical effect of the
Au/Fe/Au plasmon grating and capabilities of detecting bio-molecules. The measured
maximum of Kerr parameter is about 0.035 and a very dispersive near the SPP
wavelength. Through this integrated magneto-optics device, we have demonstrated
detection of NaCl in salt solution in low concentrations. The calculated detection of
limit is around 10-7 RIU , corresponding to a minimal concentration of 0.004% (wt).
Moreover, we investigate avidin/bBSA binding and show a result of real-time
monitoring of avidin and bBSA interaction. The minimum detectable concentration of
avidin is measured to be around 1.5nM. Our device could be fabricated by a
nanoimprinting technique for mass production and integrated with microfludic
channels for implementing a lab-on-chip system to detect bio-molecules.

並列關鍵字

Magneto-optical Kerr Effect ； Surface Plasmon ； Sensor

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