冬蟲夏草水萃液對量子點所引起之小鼠骨髓幹細胞分化抑制上的影響

Effects of Cordyceps sinensis on the inhibition of mice bone marrow cell differentiation by CdSe/ZnS quantum dots

陳孟琦(Meng-Chi Chen)

指導教授 ： 江啟勳

國立清華大學/原子科學院/生醫工程與環境科學系/碩士(2007年)

https://doi.org/10.6843/NTHU.2007.00206

摘要

冬蟲夏草是中國人長久以來食用的中醫藥材，在許多研究中都指出冬蟲夏草有調節免疫的效果，在最近的研究中也指出冬蟲夏草水萃液有促進骨髓幹細胞分化的能力。量子點是目前備受矚目的新興螢光材料，因為其螢光表現的穩定度高，目前有許多研究運用為活細胞的追蹤劑。但近年研究發現量子點在不致細胞死亡的濃度下，對於骨髓幹細胞的標定會造成細胞分子層面上的改變，而抑制幹細胞的分化。本實驗旨在探討冬蟲夏草是否能減緩或阻止量子點對骨髓幹細胞分化的抑制作用。由本論文的實驗結果可以得知，由BMP的mRNA表現量、ALP的活性表現、鈣離子的表現量都可以看出量子點對於骨髓幹細胞的分化確實有抑制的情形；而冬蟲夏草水萃液對骨髓幹細胞的分化確實有促進的效果；結合冬蟲夏草水萃液來進行骨髓細胞的量子點標定，可以有效減緩量子點對骨髓細胞所造成分化抑制的情形。此外，我們也觀察冬蟲夏草水萃液對於活化蝕骨細胞的影響，發現冬蟲夏草水萃液能夠有效降低骨母細胞的RANKL表現量，有抑制蝕骨細胞分化成熟的潛力。

關鍵字

冬蟲夏草 ； 量子點 ； 骨髓幹細胞 ； 分化

並列摘要

Cordyceps sinensis is Chinese herb medicine and is frequently used as immune regulator. Recently, Cordyceps sinensis hot-water extract has also been found to have effects on the differentiation of mice bone marrow cells. With the characteristics of high fluorescence intensity and stability, quantum dots have become the most popular fluorescent tracers in living cell imagings. However, several recent studies have shown that using quantum dots to label bone marrow stem cells could inhibit their differentiation processes. For this concern, we aim to see whether the administration of Cordyceps sinensis hot-water extract can reduce the inhibitory effect of quantum dots labeling on the differentiation of bone marrow stem cell. According to our study by measuring the mRNA expression level of BMP, the activity level of ALP and the level of calcium ions, we found that quantum dots have an inhibitory effect on mice bone marrow differentiation, which can be reduced by the administration of Cordyceps sinensis. Additionally, we also found that Cordyceps sinensis hot-water extract has opposite effect on osteoclast differentiation. We found that bone marrow stem cells treated with Cordyceps sinensis hot-water extract can reduce the mRNA expression level of RANKL in osteoblasts, which consequently reduced the osteoclast differentiation.

並列關鍵字

Cordyceps sinensis ； quantum dots ； bone marrow ； differentiation

參考文獻

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