Differential adhesion and actomyosin cable collaborate to drive Echinoid-mediated cell sorting

細胞黏著差異與肌動球蛋白索共同調控Echinoid所調節之細胞分離現象

張立勳(Li-Hsun Chang)

指導教授 ： 徐瑞洲

國立清華大學/生命科學院/分子醫學研究所/博士(2011年)

https://doi.org/10.6843/NTHU.2011.00193

摘要

Cell sorting involves segregation of two cell populations into immiscible adjacent tissues with smooth borders. Echinoid (Ed), a nectin ortholog, is an adherens junction molecule in Drosophila, and cells mutant for ed sort out from the surrounding wild-type cells. However, it remains unknown which factors trigger cell sorting. Here, we dissect the sequence of this process and find that cell sorting occurs when differential expression of Ed triggers the assembly of actomyosin cable. Conversely, Ed-mediated cell sorting can be rescued by recruitment of Ed, via homophilic or heterophilic interactions, to the wild-type cell side of the clonal interface, even when differential Ed expression persists. We found, unexpectedly, that when actomyosin cable was largely absent, differential adhesion was sufficient to cause cell segregation but with a jagged tissue border. Together, we propose that Ed-mediated cell sorting is driven both by differential Ed adhesion that induces cell segregation with a jagged border and by actomyosin cable assembly at the interface that smoothens this border.

關鍵字

細胞黏著

並列摘要

無資料

並列關鍵字

cell adhesion ； actomyosin ； cell sorting

參考文獻

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