Investigation of Spectrometer-free Surface Plasmon Resonance Sensors based on Prism Couplers

肉眼辨識稜鏡式電漿共振生物感測器之研究

張馨云(Hsin-Yun Chang)

指導教授 ： 嚴大任

國立清華大學/工學院/材料科學工程學系/碩士(2008年)

摘要

Surface plasmon resonance (SPR) biosensor enables a compelling biodetection method which is characterized by label-free detection, small sample volume required, and high sensitivity. There are four configurations of prism coupler-based SPR sensors: angular interrogation, wavelength interrogation, intensity measurement, and phase interrogation. Among the four configurations, wavelength interrogation shows outstanding sensing ability comparing with other configurations. In addition, wavelength interrogation possesses a further advantage of showing the variation in resonance wavelength that has the potential to be easily distinguished by naked eyes, rather than assisted by expensive spectrometers.
Our goal of this thesis is to further promote the sensitivity of wavelength interrogation to achieve naked eyes detections. For naked eyes observations, we consider not only the sensitivity of the system but also the sensibility of human eyes. For this reason, we conscientiously discussed the optimized thickness of metal films and the specific incident angle for detecting water solutions, the maximum content in most organisms, to reach the goal of naked eyes observations.
We used white light LED as our light source, having the following advantages, cheap, longer lifespan than traditional filament light bulb, and no overheating problem. We first demonstrated the performance of gold film detections, and observed that 43nm of gold film with 3nm of titanium adhesion layer at 62º has the best performance for detecting water solutions among other experimental parameters. Further, we displayed that silver film performs higher sensitivity than gold film. However, silver film has poor biocompatibility and extremely oxidation and sulfidation problems. Therefore, we refined our system to bilayer structure and verified that 35nm silver film plus 5nm gold film with 3nm titanium adhesion layer at 57º owns the promoted sensitivity for water solutions detection and still remains biocompatibility.
In addition, we applied the aforementioned innovative properties of SPR biosensors to investigate the binding interaction of biomolucules. A couple of high affinity biomolecules, biotin and streptavidin, were adopted to examine the functions of the home-made SPR biosensor and proved that our system can perform as biospecific binding SPR biosensors.
Currently, we utilize our system for further biomedical applications, such as drug developments, bacteria drug resistance detections, etc. We also modify our system to a portable device and improve the sensitivity for the next generation of SPR systems.

關鍵字

表面電漿共振生物感測器

並列摘要

無資料

並列關鍵字

Surface Plasmon Resonance Biosensors

參考文獻

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