有機/無機添加矽酸鈣生醫用混凝土之開發與應用研究

Development and application of biomedical concrete with organic/inorganic additives

牛慈伶(Tzu-ling Niu)

指導教授 ： 金重勳

國立清華大學/工學院/材料科學工程學系/碩士(2008年)

摘要

工業用的混凝土主要為由水、水泥及粒料(如:砂及小碎石等)組成的混合物。而本實驗研究目的即製備醫藥級的人工混凝土，應用於修復骨頭缺陷。
本實驗生醫用混凝土設計方面，以Zn0.075Ca2.925SiO5、氫氧基磷灰石( HAp )及□-三鈣磷酸鹽( □-TCP )分別替代水泥、砂及小碎石的角色。Zn0.075Ca2.925SiO5由試藥級的CaCO3、SiO2 及ZnO於1400 oC經固態反應煆燒製備，將所得的Zn0.075Ca2.925SiO5粉末再利用高速球磨細化使粒徑達10 □m以下，並以10 wt% NaH2PO4 溶液為連結劑進一步混合其它無機成份HAp及□-TCP。此外，添加生物用膠原蛋白( Collagen )及海藻酸鈉( Alginate )改善其生物相容性。此生醫混凝土無機成份比例選用Zn0.075Ca2.925SiO5 : HAp: □-TCP為1: 2: 4，並添加collgen及alginate各1 wt% (簡稱: G1)。此材料成分具有適當的操作性質、工作及固化時間，分別為11及90分鐘，且平均壓縮強度達30~40 MPa。並以純Zn0.075Ca2.925SiO5添加同G1含量高分子作為對照組(簡稱: O1)做深入研究。
生物性質測試方面，使用老鼠纖維母細胞( L929 )進行體外細胞實驗。細胞毒性測試方面，G1相對於O1顯示優越的生物相容性，且可刺激細胞分裂成長。在動物實驗方面，由一個月的骨頭外觀照片顯示G1具有不錯的骨骼修補能力。綜合上述生物實驗結果，皆顯示G1為一具有良好生物相容性的生醫混凝土材料，可用於骨骼填補。

關鍵字

三鈣矽酸鹽 ； 生醫用混凝土 ； 氫氧基磷灰石 ； 三鈣磷酸鹽 ； 膠原蛋白 ； 海藻酸鈉 ； 老鼠纖維母細胞株 ； 紐西蘭大白兔

並列摘要

Technological concrete is a construction material commonly composed of water, cement and aggregate such as sand and gravel . The purpose of this study is to fabricate biomedical concrete for bone defect repair.
In this research, the roles of the cement, sand and gravel are respectivly Zn0.075Ca2.925SiO5, hydroxylapatite( HAp ) and□□-tricalcium phosphate (□-TCP). Zn doped tricalcium silicate powders (Zn0.075Ca2.925SiO5) are prepared by calcining reagent-grade CaCO3, SiO2 and ZnO at 1400 oC through the solid-state reaction. And then the Zn0.075Ca2.925SiO5 powders are milled by high-energy ball milling to lower the particle size under 10 □m. 10 wt% NaH2PO4 used as binder, furthermore, biological polymers, collagen and alginate, are added to improve the bio-compatibility of the biomedical concrete. The inorganic component ratio of the biomedical concrete is 1 : 2 : 4, and organic additives were 1 wt% collagen and 1 wt% alginate (named : G1). The biomedical concrete mixed with NaH2PO4 solution has proper 11 minutes working time and 90 minutes setting time. The compressive strength of the biomedical concrete is averagely 30~40 MPa. Pure Zn0.075Ca2.925SiO5 added with the same polymer content is prepared and serving as relative ( named : O1). G1 and O1 are further studied.
Mice fibroblast cell line ( L929 ) is cultured for in vitro test. In the cytotoxicity tests, G1 shows excellent bio-compatibility comparing to O1. In addition, for Animal test for Newland white rabbits in vivo, G1 shows good repairing ability.

並列關鍵字

Tricalcium silicate ； bioconcrete ； HAp ； -TCP ； collagen ； alginate ； L929 ； Newland white rabbits

參考文獻

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