摘要
流式細胞儀乃生醫領域中一重要之前端基礎儀器，其功能為快速將細胞篩選分類並精準計數，對免疫檢測等領域極有貢獻。本研究創新使用微形巨磁阻感測器，藉由量測磁感測器之磁阻變化快速將磁標定之細胞計數，並在計數後將其分離收集。 本文所設計之磁阻式流式細胞儀分為三大部份，包括前端細胞流的水力聚焦、中段的磁細胞偵測、及後端的磁細胞分類收集，重點為磁細胞偵測部份。本文設計多種巨磁阻感測器，探討不同形狀磁膜對磁細胞偵測靈敏度的差異，發現形狀異向性較小的磁膜較有利於計數細胞，其感測器靈敏度約為5.4×10-3 Ohm/Gauss。除此之外，分辨出胞吞不同磁顆粒數量的磁細胞亦為此部份中心目標之一。在後端收集部份，本文初步針對使不同流率之細胞流發生流向偏折所需的外加磁場進行數據建立，為日後自動化的工作打下基礎。
並列摘要
Flow cytometry is a front-end basic instrument in biomedical field. The flow cytometry can count and sort cells fast and precisely, and contributes greatly in areas such as immunoassay. In this study, we innovatively use micro Giant Magnetoresistive (GMR) sensor to detect cells. By measuring the magnetoresist variations induced by the magnetic-marked cells, the number of magnetic cells passed through the sensor can be counted. Besides, we also sort the magnetic cells magnetically after the counting. Our magnetoresistive flow cytometry is composed of three parts including microfludic part for hydrodynamic focusing of cell stream, spintronic part for cell detecting, and cell separation and sorting part. The most important part is the cell detecting. In this part, GMR sensors are designed in different shapes to test their sensitivity for magnetic cell detecting. From the experiment results, we found a sensor have smaller shape anisotropy is a better choice to be used to count cells. In addition to counting cells, the sensor also need to distinguish cells which endocyte different numbers of magnetic particles. In the separation and sorting part, the threshold fields for making cells’ flow direction bending adequately are found at different flow velocities. It will be useful for future automation work.
並列關鍵字
無資料參考文獻
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