利用環型流道及液胞形成技術於微藻分離之應用

Cell-in-Droplet Encapsulation by Using Dean Flow in Spiral Microchannel Device for Microalgae Separation

李明蓉(Porntipa Sankatumvong)

指導教授 ： 饒達仁

國立清華大學/工學院/奈米工程與微系統研究所/碩士(2016年)

摘要

In this study, a cell-in-droplet encapsulation using dean flow in spiral microchannel device is applied for microalgae separation. Researchers are interested in separating microparticles by using microfluidic chips in recent years due to great advantages for variety kinds of related applications such as biotechnology, medical examinations, or cell studies. However, the main disadvantage of these microfluidic chips is that it usually would experience particles clogging which reduces the separation yield and hard for particles investigation. The microfluidic chip being introduced in this study is a combination of 2 distinct designs: (1) spiral microchannel design used for separating different sizes of microalgae and (2) microdroplet generation design used for cell encapsulation. The reason is to enhance the separation yield by using different dominant forces concept (Dean drag force and lift force) in spiral microchannel design together with microdroplet generation design narrow down the volume for easier cell observation.
The microfluidic chip was fabricated by using soft lithography techniques. Polydimethylsiloxane (PDMS) is well known as biocompatible material, low cost of production, disposable, more over it is transparency makes it possible to observe particles inside the microchannel clearly. Due to all of these benefits, this device might be an alternative for cell applications using droplet-based platforms.

關鍵字

cell separation ； microdroplets ； microalgae ； pdms ； cells in droplets

並列摘要

In this study, a cell-in-droplet encapsulation using dean flow in spiral microchannel device is applied for microalgae separation. Researchers are interested in separating microparticles by using microfluidic chips in recent years due to great advantages for variety kinds of related applications such as biotechnology, medical examinations, or cell studies. However, the main disadvantage of these microfluidic chips is that it usually would experience particles clogging which reduces the separation yield and hard for particles investigation. The microfluidic chip being introduced in this study is a combination of 2 distinct designs: (1) spiral microchannel design used for separating different sizes of microalgae and (2) microdroplet generation design used for cell encapsulation. The reason is to enhance the separation yield by using different dominant forces concept (Dean drag force and lift force) in spiral microchannel design together with microdroplet generation design narrow down the volume for easier cell observation.
The microfluidic chip was fabricated by using soft lithography techniques. Polydimethylsiloxane (PDMS) is well known as biocompatible material, low cost of production, disposable, more over it is transparency makes it possible to observe particles inside the microchannel clearly. Due to all of these benefits, this device might be an alternative for cell applications using droplet-based platforms.

並列關鍵字

microdroplets ； microalgae ； pdms ； cells in droplets ； cell separation

參考文獻

CHAPTER VI REFERENCES

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[2] J. Giddings, "Field-flow fractionation: analysis of macromolecular, colloidal, and particulate materials," Science, vol. 260, pp. 1456-1465, June 4, 1993 1993.

[3] E. Chmela, R. Tijssen, M. T. Blom, H. J. G. E. Gardeniers, and A. van den Berg, "A Chip System for Size Separation of Macromolecules and Particles by Hydrodynamic Chromatography," Analytical Chemistry, vol. 74, pp. 3470-3475, 2002/07/01 2002.

[4] M. Yamada, M. Nakashima, and M. Seki, "Pinched Flow Fractionation:  Continuous Size Separation of Particles Utilizing a Laminar Flow Profile in a Pinched Microchannel," Analytical Chemistry, vol. 76, pp. 5465-5471, 2004/09/01 2004.