交流電滲流輔助高密度的同步集中SERS奈米粒子和生物分子應用於快速生物檢測

一般常見的生物檢測方式有：一、聚合酵素連鎖反應 (Polymerase Chain Reaction，PCR)。二、酵素連結抗體吸附反應(Enzyme-linked Immunosobent Assay，ELISA），但此類方法最大的問題在於需要大量時間才能取得結果，無疑不利於急需結果之檢體。而表面增強拉曼散射（SERS）所能提供的即時性、特定專一性高、不需標定、可遠距離觀測、對檢體相對少的物理及化學破壞等優點，促使大量研究團隊扎根於此。　　本研究之表面增強拉曼散射（SERS）方法建構於兩個機制：一、表面增強拉曼散射粒子（AuPSBs or AgPSBs）。二、交流電滲流晶片。表面增強拉曼散射粒子（AuPSBs）是將聚苯乙烯奈米球經過氧電漿轟擊使之形成奈米粗糙表面，並於此表面鍍上金屬膜，與不具有奈米粗糙表面的金屬鍍膜粒子相比約有5倍的拉曼訊號增強能力；交流電滲流晶片是利用調控電壓及頻率達到操控懸浮粒子的電動力學，此方法可以在2分鐘內提高10倍的訊號強度，且交流電滲流可控制懸浮粒子集中的位置，此法可一併解決拉曼散射檢測中待測目標物定位上的困難。將以上兩種機制結合後，與僅靠金球的SERS相比則可以在2分鐘內達到50倍的訊號放大能力，未來應可實用在生物或醫學的快速檢測上

關鍵字

交流電滲流；表面增強拉曼散射；生物檢測

並列摘要

General common biological detection: 1st Polymerase Chain Reaction, PCR). 2nd Enzyme-linked Immunosobent Assay, ELISA). but such methods biggest problem that requires a lot of time to get results, certainly not conducive to the urgent need of the results of the inspection body. The high surface-enhanced Raman scattering (SERS) can provide immediate, specific specificity without calibration, remote observation, the advantages of the specimen relatively few physical and chemical damage, prompting a lot of research team has focus it . The study of surface-enhanced Raman scattering (SERS) method to construct two mechanisms: 1st Surface-enhanced Raman scattering particles (AuPSBs or AgPSBs). 2nd AC Electroosmotic Chip. AuPSBs is etching polystyrene beads formation of nano-rough surface by oxygen plasma bombardment and deposit metal film on the surface, and does not have a the nano rough surface of metal coated particles Raman signal compared to about 5 times capacity; AC seepage chip is the regulation of voltage and frequency of suspended particulates manipulation electrodynamics, this method can be a tenfold improvement in signal strength within six minutes, and AC seepage can control the suspended particulates centralized location, this method can be resolved Raman scattering detection in the object to be tested positioning difficulties. Combining the above two mechanisms can be achieved in 2 minutes 50 times signal amplification capability, should be in the future utility in the rapid detection of biological or medical.

並列關鍵字

AC-ELECTROOSMOSIS ； SERS ； BIO-DETECTION

參考文獻

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[2]. J. W. McMurdy III, A. J. Berger, “Raman Spectroscopy-Based Creatinine Measurement in Urine Samples from a Multipatient Population”, Applied Spectroscopy 57, 5, 2003

[3]. R. L. McCreery, “Raman Spectroscopy for chemical analysis”, New York: Wiley Interscience, 2000

[4]. M. Fleischmann, P. J. Hendra, A. J. McQuillan, "Raman Spectra of Pyridine Adsorbed at a Silver Electrode", Chemical Physics Letters, 26, 163–166, 1974

[5]. M. Moskovits, “Surface roughness and the enhanced intensity of Raman scattering bymolecules adsorbed on metals”, J. Chem. Phys., 69, 1459-1461, 1978

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交流電滲流輔助高密度的同步集中SERS奈米粒子和生物分子應用於快速生物檢測

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