- 學位論文
重組鱟血漿凝集素之細菌及配體結合功能分析
Functional Caracterization of Bacteria and Ligand Binding Activities of Recombinant Tachypleus Plasma Lectin
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摘要
中文摘要 鱟是古老的無脊椎海洋動物，缺乏後天性免疫系統，因此完全依賴由血漿及血球組成的先天性免疫系統抵抗病原菌入侵。由台灣品種鱟 (Taiwanese Tachypleus tridentatus) 的血漿中分離之血漿凝集素具有辨識革蘭氏陽性和陰性菌、以及其他病源菌相關分子型態(pathogen associated molecular patterns, PAMPs) 的能力。以往利用嗜甲醇酵母菌 (Pichia pastoris) 可於胞外表現鱟血漿凝集素，但其表現量及產率皆低；而目前尚無利用原核生物系統表現並純化可溶性鱟血漿凝集素的成功案例。本研究利用蛋白質工程技術設計及修飾重組鱟血漿凝集素胜肽序列，並利用大腸桿菌表現此新穎之重組蛋白。其生物功能性分析顯示重組鱟血漿凝集素可識別特定的細菌及病原相關分子型態、亦能結合多種臨床分離之病源菌，並發現此結合細菌的能力可能與病源菌細胞壁中特殊醣分子相關。此外，本研究亦首次發現重組鱟血漿凝集素具有抑制綠膿桿菌生長的能力。本論文探討重組鱟血漿凝集素與細菌、病原菌及糖類的特定分子交互作用關係，將有助於發展偵測臨床微生物致病菌或內毒素殘留的新穎方法。
關鍵字
鱟血漿凝集素參考文獻
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延伸閱讀
- Ling, L. E. (2010). 重組鱟血漿凝集素2之細菌及配體結合活性探討 [master's thesis, National Tsing Hua University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0016-1901201111393653
- Hao, H. C. (2017). 利用重組鱟血漿凝集素識別及移除細菌內毒素 [master's thesis, National Tsing Hua University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0016-0401201815532934
- En, C. Y. (2015). 重組鱟血漿凝集素之細菌及配體結合位與抑菌功能鑑定 [master's thesis, National Tsing Hua University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0016-0312201510242350
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