- 學位論文
石墨烯電晶體單液滴式快速檢測細菌
Graphene transistor-based biosensor for single-droplet rapid detection of bacteria
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摘要
台灣位於亞熱帶地區，每逢夏季食物中毒案件頻傳。民國一百年衛生署統計，該年度有兩千七百餘人因食品遭細菌汙染而中毒。針對細菌檢測，衛生署雖訂定「食品微生物之檢驗方法－生菌數之檢驗」法，但總生菌量之檢測流程須24小時，無法在民眾飲食前確認食品的安全與否。 本研究提出一萬古黴素-大面積氧化石墨烯電晶體(VLGO)快速細菌之生物感測器，具備快速、可重複使用與低檢測量的優點。我們以化學沉積法(CVD)生長大面積石墨烯，以聚對苯二甲酸乙二酯(Polyethylene terephthalate, PET)為基板，並透過氧電漿改質為帶有含氧官能基的大面積氧化石墨烯(LGO)，最後透過橋接分子在其表面修飾萬古黴素。 VLGO為一p型電晶體，當注入10微升的待測液至VLGO表面時，萬古黴素便以氫鍵抓取溶液中的細菌。帶負電的細菌使VLGO表面電洞數量增加，導致導電度上升。我們可藉由量測VLGO導電度改變量而得知細菌濃度。 本研究以大腸桿菌與金黃色葡萄球菌作為測試，其偵測極限分別為11 cfu/ml及16 cfu/ml，反應時間為十五分鐘。透過本研究所提出的細菌偵測機制，能成功突破傳統檢測方式之缺點，有效於短時間內檢測細菌濃度，降低食物中毒發生的機會。
並列摘要
Food poisoning is an obstinate global issue. The rapid detection of pathogens before food intake can be a good strategy to avoid being infected. However, conventional methods usually take more than two days for pathogens incubation and detection. Vancomycin can capture the bacteria using the linkage of five hydrogen bonds between the heptapetide backbone and the D-alanyl-D-alanine dipeptide extending from the cell wall of bacteria. In this study, we develop a rapid bacteria detection device based on the vancomycin modified large graphene oxide (VLGO) transistor. At first, a single-layered graphene was prepared by chemical vapored deposition method3 and transferred onto PET as the substrate. Second, we modified the surface by oxygen plasma treatment. Further, the surface of graphene was functionalized with vancomycin. Finally, the pads of source and drain were deposited onto the VLGO surface. The device is demonstrated to be simple, rapid and reusable bydropping a 10µL solution onto surface of VLGO followed by applying a voltage bias between source/drain. As a p-type transistor, the density of the holes on VLGO surface increases once the bacteria were captured by vancomycin. The detection readout is obtained by comparing the conductance before and after the applying the sample solution onto the VLGO transistor. The results demonstrated that the detection limit is 16 cfu/ml, 11cfu/ml for S.auresus and E.coli, respectively. The response time is 15 minutes. The device is often rapid detecting bacteria and a promising candidate to overcome the hurdles encountered by conventional methods care of food poisoning.
並列關鍵字
無資料參考文獻
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