應用縮時螢光顯微鏡影像的老鼠誘發性多功能幹細胞生成之偵測與定位研究

Detection and Localization of Mouse Induced Pluripotent Stem Cell Formation using Time-Lapse Fluorescence Microscopy Images

劉融諴(Jung-Hsien Liu)

指導教授 ： 張元翔 ； 蔡明達

中原大學/電機資訊學院/通訊工程碩士學位學程/碩士(2016年)

https://doi.org/10.6840/cycu201600357

摘要

我們呈現一個自動化的方法，通過使用螢光顯微鏡影像，來偵測與定位老鼠誘導性多能幹細胞（iPS細胞）的形成。透過篩查試劑或培養條件的方法來誘導已分化細胞可能使細胞重新生成為多功能性幹細胞。我們提出的方法包括: 影像前處理，以強化螢光顯微鏡影像，接著是細胞偵測，偵測單獨的螢光細胞（Reprogramed或Reprograming的iPS細胞）。最後，群聚技術，偵測和定位螢光顯微鏡細胞的群聚。實驗結果顯示，這個自動化的方法能夠成功偵測和定位老鼠誘導性多功能幹細胞之形成，是個很有潛力的工具來幫助老鼠誘導性多功能幹細胞的培養。

關鍵字

電腦視覺 ； 螢光顯微鏡影像 ； 細胞偵測 ； 誘導性多功能幹細胞

並列摘要

Abstract—We present an automated method for detection and localization of mouse Induced pluripotent stem (iPS) cells formation by using the fluorescence microscopy images. The differentiated cells that possibly undergo reprogramming to iPS cells can be detected by this method for screening reagents or culture conditions in iPS induction. Our method includes image preprocessing to enhance the fluorescence microscopy images, followed by cell detection to detect isolated fluorescent (reprogramming or reprogrammed iPS) cells. Finally, a clustering technique is used to detect and localize the fluorescent cell clusters. A cluster formation indicates that the reprogramming iPS cells may become reprogrammed. The implementation results show that the automatic method can successfully detect and localize the mouse iPS cell formation, thereby yield a potential tool for helping iPS cell culture.

並列關鍵字

computer vision ； fluorescence microscopy images ； cell detection ； induced pluripotent stem cells

參考文獻

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