免疫感測器與積體化溫度感測元件之製作

Fabrication of the Immunosensor and Integrated Temperature Sensor

范錦達(Chin-Ta Fan)

指導教授 ： 熊慎幹

中原大學/工學院/電子工程研究所/碩士(2003年)

https://doi.org/10.6840/cycu200300286

摘要

低密度脂蛋白及高密度脂蛋白係負責人體中膽固醇之代謝與運輸，於臨床診斷上，低密度脂蛋白膽固醇及高密度脂蛋白膽固醇之濃度係判斷冠狀動脈心臟疾病重要參數之一。
本論文係利用分離架構之延伸式SnO2/ITO glass離子感測場效電晶體製作低、高密度脂蛋白膽固醇免疫感測器，並探討各參數對整體反應之影響，尋找出最佳化之工作條件。經由實驗得知此架構之低密度脂蛋白膽固醇免疫感測器於5mM、pH7.4、25℃ 的磷酸鹽緩衝溶液中其偵測線性範圍介於130-170mg/dL；高密度脂蛋白膽固醇免疫感測器於5mM、pH7.4 之磷酸鹽緩衝溶液中的偵測線性範圍介於35-75 mg/dL。
另一方面，吾人利用UMC 0.5um之CMOS標準製程設計製作一積體化溫度感測元件，並對此溫度感測元件進行溫度特性之模擬與量測，以監控生物感測元件操作及保存時之溫度，給予溫度資訊，進行溫度補償，增加生物感測元件之準確度。此溫度感測元件之工作電壓為3V，溫度工作範圍係介於15~45℃。

關鍵字

延伸式離子感測場效電晶體 ； 低密度脂蛋白 ； 高密度脂蛋白 ； 溫度感測元件 ； 免疫感測器

並列摘要

Low density lipoprotein(LDL) and high density lipoprotein(HDL) are known to play a central role in cholesterol transport and metabolism. In clinical diagnosis, the concentration of the low density lipoprotein cholesterol(LDL-C) and the high density lipoprotein cholesterol(HDL-C) in the blood are the important parameters for diagnosing heart disease.
In this study, the extended-gate field effect transistor of the SnO2/ITO glass was applied to fabricate the LDL-C and the HDL-C immunosensors. In order to realize the optimal measurement environment and the best response curves, the influence of the response was conferred. According to experimental results, the separative structure of the LDL-C immunosensor had a dynamic range from 130 to 170 mg/dL that was measured at 5mM phosphate buffer solution in pH 7.4. The HDL-C immunosensor had a dynamic range from 35 to 75 mg/dL that was measured at 5mM phosphate buffer solution in pH 7.4. This configuration of the LDL-C and the HDL-C immunosensors have many advantages, such as the low cost, simple frame, and easy to measure.
In addition, the CMOS standard process of the UMC 0.5um was employed to fabricate the temperature sensor. The temperature sensor was designed for biomedical applications. Utilizing the temperature sensor monitor the measurement and reserve temperature of the biosensor to increase the reliability of the biosensors. The temperature sensor has a dynamic range between 15 and 45℃ and the optimal supply voltage is 3V.

並列關鍵字

Low Density Lipoprotein ； Extended-gate Field Effect Transistor ； Temperature Sensor ； Immunosensor ； High Density Lipoprotein

參考文獻

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55. 邱嘉政, “延伸式閘極離子感測場效電晶體穩定度之研究”, 中原大學電子工程研究所碩士論文, pp. 19-21, 2001.

58. 李秀文,“新型膽固醇生醫感測器之研究”, 中原大學醫學工程研究所碩士論文, p. 10, 2001.

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