- 學位論文
以延伸式場效電晶體製作銨根離子感測元件
Ammonium Ion Sensitive Electrode Based on the Extended gate Field Effect Transistor Structure
摘要
由於血液中之尿素濃度可反應蛋白質的攝取及異化作用，尿素係哺乳類動物蛋白質代謝之主要產物，攝入蛋白質食物愈多，產生之尿素亦愈多，同時與腎臟、肝臟功能及腎上腺內分泌機能有密切之關聯，係臨床診斷用以判斷腎功能之重要參數。 本論文係利用分離架構之延伸式SnO2/ITO glass離子感測場效電晶體製作一種耐用型的銨根離子選擇電極，並探討各個參數對整體反應之影響，如改變不同添加劑、添加不同高分子物質、改變量測環境之起始pH值、改變緩衝溶液之濃度…等。經由一連串之實驗結果得知此一銨根離子選擇電極於0.5 mM Tris / EDTA pH 7.5之工作環境下有最佳反應曲線，可偵測銨根離子濃度10-5 ~ 1M之範圍，線性部分之平均感測度為47.64 mV / pNH 。 另一方面，銨根離子選擇電極必須強調其穩定性與再現性之機制，又離子干擾之影響亦係離子選擇電極的重點。故須找出其最佳化的量測環境來製作一穩定之銨根離子選擇電極。之後，才能發展出可長期使用的電極。
並列摘要
In blood the urea concentration can response the unusual absorption of the protein. The main product was the protein metabolism of the mammalian in the urea. And the more we ingest protein the more urea was produced. At the same time, it was also related to the renal function and the important parameter of the clinical diagnosis. In this study, the extended-gate field effect transistor of the SnO2/ITO glass was applied to fabricate the durable ammonium ion selective electrode and confer with the influence, including the different additive, added different polymer, the pH value, the concentration of the buffer solution…etc. After a series of the experiments, the optimized measurement environment of the ammonium ion selective electrode was in 0.5mM Tris/EDTA pH 7.5 and the best response curves can be gotten. The ammonium ion selective electrode structure can detect the ammonium ion concentration from 10-5 ~ 1M, and sensitivity of the linear range is about 47.64 mV/pNH . In addition, the ammonium ion selective electrode must emphasize the stability and reproducibility. The emphasis of the ion selective electrode also include the ion interference. Therefore, we must find out the optimize measurement environment to fabricate a stable ammonium ion selective electrode. After that, the long-term used electrode can be developed.