甲醇燃料電池於細胞培養箱上之整合應用與探討

System integration of fuel cell and cell incubator

李得民(Dermine Lee)

指導教授 ： 婁世亮

中原大學/工學院/生物醫學工程研究所/碩士(2008年)

https://doi.org/10.6840/cycu200900663

摘要

本論文整合完成一套環保細胞培養系統。該系統大致可分為直接甲醇燃料電池模組及細胞培養箱模組。兩者均設計有以單晶片MSP430F1611為核心的控制電路。系統中甲醇燃料電池模組之燃料電池是將化學能轉換為電能，其控制電路則是調控周邊電路變化電壓負載、電能儲存等功能。細胞培養箱模組中含有一40 x 25 x 18 cm3的細胞培養箱和一感測控制電路，培養箱中的CO2、濕度及溫度即是藉這感測控制電路調控之。 論文中，以探討燃料電池的物理與化學特性與驗證細胞培養箱的功能性為重點。在燃料電池模組實驗方面，當輸出負載為7.2、8.1、9.0及10 V時，可使用之電流從1400 mA隨負載電壓上升而下降至350 mA。然而，當負載電流越高，電池可使用的時間越短。在副產物CO2方面，它的產生也隨著負載電壓增加而下降，如當負載為7.2 V時有50 sccm的CO2產生，然而當負載高達10 V時CO2的產生則下降至3 sccm。在檢驗儲存槽回流水之甲醇含量實驗方面，本研究運用氣相層析質譜儀完成一檢量線，藉以比對經加熱10 %體積比之甲醇水，結果顯示這甲醇水加溫至90 ℃維持四個小時可以得到接近純水。 本研究發展的溫控系統在30分鐘內可以將培養箱控制在37±0.1 ℃的範圍。濕度控制系統在8分鐘之內可以將培養箱控制在95±1 %相對濕度的範圍。CO2控制系統則在3.2分鐘之內將培養箱內的CO2濃度控制在5±2 %的範圍。本系統與市售細胞培養箱共同培養小鼠巨噬細胞，MTT測試結果顯示本系統的功能性約為市售系統之96 %。

關鍵字

直接甲醇燃料電池 ； 溫度控制 ； 濕度控制 ； 細胞培養箱

並列摘要

In this course, we have developed an environmental protection based cell culture incubator, which mainly includes a direct methanol fuel cell (DMFC) system and a cell culture system. Each of these systems is coordinated by a control module centered on a microchip, MSP430F1611.
The DMFC system includes a fuel cell pack serving as an energy conversion device of methane to electricity. The control module in the DMFC system is used to regulate voltage load, energy storage etc. The cell culture system is composed of an acrylic chamber with the size of 40 x 25 x 18 cm3 and a sensing circuit. This circuit detects and manages the concentrations of CO2, humidity, and temperature of the chamber.
The fuel cell pack was applied by a series of voltage loads to examine how the voltage load affects the applications of the fuel cell. When the loads of 7.2、8.1、9.0 and 10 V were used, the applicable currents generated by the fuel cell pack decreased from 1400 to 350 mA. Yet, the production of CO2 is inversely proportional to the voltage load used. Importantly, when high electrical current is used, the allowed usage duration of the fuel cell reduces.
In this course, the design is to apply the water from the fuel cell to supply humidity for the cell culture chamber. Unfortunately, the water is always mixed with methane that was not fully utilized by the DMFC pack. Thus, it is important to develop a method to eliminate methane from the mixture. Our approach is to steam the mixture because the boiling point of methane is about 36 oC lower than water. Our experiment results indicate that in order to obtain pure water from 10% methane water it requires boiling the mixture for four hours at 90 oC.
The developed control module associated with the cell culture system
is able to supply the temperature, humidity and CO2 concentration required in the chamber. It heated up the chamber from the room temperature to 37±0.1 oC less than 30 minutes, reached the humidity level of 95±1 % within 8 minutes, and maintained the CO2 concentration in the range of 5±2 % in 3.2 minutes. The feasibility of using the developed chamber to culture cells was evaluated by comparing its cell culture performance with that of a commercial CO2 incubator. The cell line of RAW264.7 was used in the comparison study and the MTT assay was applied to evaluate cell survival rate. The results show that the amount of the cells cultured in the developed chamber is about 96% of the commercial incubator.

並列關鍵字

Direct Methanol Fuel Cell (DMFC) ； Cell Incubator ； Tempera

參考文獻

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