- 學位論文
不同複方食材對高果糖誘發代謝症候群大鼠之影響
Effects of the Various Combinatorial Foods on Metabolic Syndrome in Fructose-Fed Rats
摘要
代謝症候群是一種許多危險因子叢聚在一起的症狀，包含胰島素阻抗、內臟脂肪堆積、高血壓以及與糖尿病及心血管疾病有關之危險因子，如脂質及脂蛋白代謝異常，內皮細胞功能異常與發炎之因子產生等。代謝症候群在未來二十年內將成為心血管疾病及糖尿病流行率的指標。目前沒有一種單一食材能全面性的預防及改善與代謝症候群有關的多重危險因子，在本實驗中，將評估不同複方食材改善高果糖誘發代謝症候群大鼠之功效。 實驗將雄性SD大鼠分成四個組別，分別是空白組 (B)：餵食Chow diet；控制組 (C)：餵食高果糖飼料；食材處理組1 (T1)：除了餵食高果糖飼料外，並添加紅麴、大豆、苦瓜、甘草及綠藻；食材處理組2 (T2)：除了餵食高果糖飼料外，並添加紅麴、鰹魚萃取物、兒茶素及豆豉萃取物。本實驗持續進行16週。實驗期間分析血壓、禁食血漿血糖、胰島素、三酸甘油酯及膽固醇，並進行口服葡萄糖耐受性測試，觀察大鼠胰島素敏感性之變化。同時亦分析胰島素訊息傳遞相關蛋白在脂肪組織上的表現。 實驗結果顯示，C組之大鼠餵食高果糖造成禁食血漿葡萄糖、胰島素、三酸甘油酯、膽固醇濃度及收縮壓顯著增加。另外在口服葡萄糖耐受性測試中，發現C組大鼠之血漿葡萄糖與胰島素濃度之曲線下面積皆顯著大於B組。此現象與大鼠餵食高果糖後氧化壓力增加、胰島素受體受質-1及葡萄糖轉運蛋白4減少有關。 相較於C組，T1組之收縮壓、禁食血漿膽固醇和葡萄糖耐受性皆有改善的現象。另外在胰島素訊息傳遞蛋白方面，T1組之胰島素受體受質-1表現有增加的趨勢。而餵食T2組食材之大鼠，其收縮壓、禁食血漿三酸甘油酯、膽固醇濃度及胰島素敏感性皆有明顯改善。探討其機制，T2組之胰島素敏感性增加，原因可能與氧化壓力減少以及葡萄糖轉運蛋白4表現增加有關。
並列摘要
Metabolic syndrome (MS) is characterized by insulin resistance, visceral obesity, and hypertension as well as by a cluster of diabetic atherosclerosis risk factors including disturbed lipid and lipoprotein metabolism, endothelial dysfunction, and elevated pro-inflammatory cytokines. MS is likely to have a marked impact on the prevalence of cardiovascular disease and type 2 diabetes worldwide in the next two decades. It is evident that MS involves multiple risk factors and any single health food may not be effective in the prevention and improvement of MS. The objective of this study was to evaluate the effects of the supplementation of the various combinatorial foods on metabolic syndrome in a fructose-fed rat model. The male SD rats were divided into four group: blank group (B), fed with standard Purina chow; control group (C), fed with high fructose diet alone; treatment 1 group (T1), fed with high fructose diet plus red yeast rice, soybean, bitter melon, licorice and green algae; treatment 2 group (T2), fed with high fructose diet plus red yeast rice, katsuobushi oligopeptide, catechin, and touchi extract. The study lasted for 16 weeks. Biochemical parameters related to blood pressure, fasting plasma glucose, insulin, triglyceride and cholesterol were measured, and an oral glucose tolerance test (OGTT) was performed in this study. The contents of insulin signaling pathway related proteins in adipose tissue were also determined. Our data showed that, high fructose feeding cause significant increased in fasting plasma glucose, insulin, triglyceride, cholesterol and systolic blood pressure in group C. And the total area under curves for glucose and insulin in OGTT significantly increased in group C as compared with group B. To further investigated the possible mechanism of the metabolic defects induced by high fructose diet, we found that the rats showed increased oxidative stress and decreased expressions of insulin signaling pathway related protein, including insulin receptor substrate-1 and glucose transporter IV. Compared with group C, systolic blood pressure, fasting plasma cholesterol and glucose tolerance were improved in group T1. And the total content of insulin receptor substrate-1 was raised in T1 group. The systolic blood pressure, fasting plasma triglyceride, cholesterol and glucose tolerance of rats in group T2 were ameliorated as well. The increased insulin sensitivity might result from the decreased oxidative stress and elevated expression of glucose transporter IV.
參考文獻
延伸閱讀
- 顏肇均（2009）。不同果糖比例飼料誘發大鼠代謝症候群現象之探討〔碩士論文，中原大學〕。華藝線上圖書館。https://doi.org/10.6840/cycu200900130
- 楊恆叡（2014）。沒食子酸(gallic acid)對高果糖飼料誘導高血糖大鼠血糖及脂質代謝之影響〔碩士論文，國立臺灣師範大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0021-0801201418031389
- 陳雅妍（2013）。不同昇糖指數之根莖主食類對於高血糖及胰島素阻抗大白鼠其體內胰島素敏感性及胰島素訊息傳遞路徑之影響〔博士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1007201310221000
- 蔡蕙芸（2007）。龍眼花水萃物對高果糖誘發代謝症候群大鼠之影響〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2007.02445
- 吳夢婷（2008）。Effects of Phytoestrogenic Foods/Herbs on Metabolic Syndrome and Ca Metabolism in Ovariectomized Mice〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2008.03077