設計螢光金奈米團簇載體用於細胞標定之研究

Design of Carrier Using Fluorescent Gold Nanoclusters for Cells Labeling

游婉淳(Wan-Chun Yu)

指導教授 ： 張恒雄

中原大學/工學院/生物醫學工程研究所/碩士(2010年)

https://doi.org/10.6840/cycu201000778

摘要

細胞標定可提供觀察細胞或活體動物內交互作用的機制，但細胞自發性螢光蛋白的影響，會造成很高的背景雜訊，而有機染料和量子點可避免這個缺點，但它們分別有光漂白和細胞毒性等問題，因此，發展較不具毒性之螢光材料成為當今首要之課題。金奈米粒子不具螢光特性，但當粒徑小於一定奈米尺度時，其特性改變且產生不連續之電子能階，會改變原本金屬特性而產生量子侷限效應，此時僅有少數金原子所組成之金奈米團簇( Gold nanoclusters, AuNC )便具有螢光特性，且被認為具有良好之生物相容性、化學穩定性及表面修飾等特性，可成為新一代之螢光標定物質，發展螢光金奈米團簇之合成製備方式已成為目前重要研究發展之方向。本研究利用牛血清白蛋白(BSA)進行水溶性螢光金奈米團簇的合成，為了改善螢光金奈米團簇進入細胞的能力，利用兩種不同載體設計，分別為帶正電脂質團(LipofectamineTM 2000 Reagent)與氧化鐵，使螢光金奈米團簇帶有正電荷與磁性功能，探討電性與磁性功能是否可以增加螢光金奈米團簇進入到細胞的能力，從顯微鏡觀察可以得知，經過載體設計後，迎光金奈米團簇可以標定到細胞中，顯著提升在細胞中觀察螢光金奈米團簇的螢光表現，並且由共軛焦顯微鏡之Z軸截面，可以證實螢光金奈米團簇確實進入細胞中而非貼附於細胞表面上。

關鍵字

奈米團簇 ； 載體 ； 細胞 ； 標定 ； 螢光金

並列摘要

Semiconductor quantum dots are attracted by its stability and tunable wavelengths, but containing toxic ions such as Cd2+, Pb2+ is a concerned issue for broad clinical application. To face on this problem, researchers have focused on developing new biocompatible materials with fluorescent properties. Fluorescent gold nanoclusters are becoming the alternative nanomaterials for nontoxic cellular labeling. Gold nanoclusters, consisting of several atoms, exhibit discrete electronic states and fluorescent properties. As a biocompatible materials, gold nanoclusters show a good candidate of novel fluorophore with many advantages, such as chemical stability, and general surface chemistry. In this study, synthesis of ultrafine fluorescent gold nanoclusters is included in this report. We focus on the issue how to efficiently label cells using specific carrier. we study the cell labeling efficiency of fluorescent gold nanoclusters using different forces (electric, megnetic). The nanoprobes design of fluorescent gold nanoclusters are also included. Specific staining of cells and nonspecific uptake by living cells are studied.

並列關鍵字

Carriers ； Fluorescent Gold ； Nanoclusters ； Cell ； Labeling

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