具溫度響應之雙離子性醋酸纖維膜於可逆性細菌貼附控制之探討

Thermoresponsive Cellulose Acetate Membrane Containing Polyzwitterions for the Reversible Control of Bacterial Attachment and Biofilm Formation

黃國倫(Kuo-Lun Huang)

指導教授 ： 張雍

中原大學/工學院/化學工程研究所/碩士(2010年)

https://doi.org/10.6840/cycu201000835

摘要

發展具有生物惰性與生物活性調控功能表面的智慧材料，是現今主要生醫材料研究課題。而在本研究主要以溫感性的高分子(N-isopropylacrylamide)與雙離子性高分子(zwitterionicsulfobetaine methacrylate) 以低壓電漿聚合法接枝於醋酸纖維膜中(cellulose acetate)，以成為可調控之生物感應膜。並製備出混合式與雙層結構的改質膜，以探討微生物吸附之可逆效果。表面化學組成與性質鑑定以傅立葉紅外線光譜儀(Fourier Transform Infrared Sepectromter)、表面化學分析儀(X-ray photoelectron spectroscopy)、接觸角量測儀(contact angle) 、與掃描式電子顯微鏡(Scanning Electron Microscopy)觀察改質膜材之定性與定量分析。改質膜於生物環境中展現出高的溫度感應性、膨潤度與親水能力以達到調控大腸桿菌(Escherichia coli)的貼附行為。而對於非離子性的NIPAAm含量較高的接枝膜，由於在高溫中展現出疏水特性，以致含有較高的細菌貼附。反之SBMA含量高的接枝膜，藉由表面的親水特性，展現出抗微生物沾黏之特性。而本研究以雙層結構探討微生物的可逆吸脫附效果，發現可有效控制微生物脫附速率。這種藉由環境感應而調控表面特性的改質膜，對於智慧材料的生物應用上，有很大貢獻與發展。

關鍵字

電漿改質法 ； 三醋酸纖維膜 ； 智能抗菌膜 ； 抗生物沾黏 ； 低臨界流體溫度

並列摘要

A great challenge in the smart biomaterials is to design a dual-functional bioinert/bioactive surface with reversible bioadhesive property. This work describes a novel tunable bioadhesive membrane of cellulose acetate (CA) grafted with thermoresponsive N-isopropylacrylamide (NIPAAm) and zwitterionic sulfobetaine methacrylate (SBMA). Two grafting structures of PNIPAAm-SBMA copolymer layer, mix- and bi-layer grafting, were controlled using low pressure plasma-induced graft polymerization and varing NIPAAm and SBMA molar ratios. The chemical composition and microstructure of the various surface-modified CA membranes were characterized by Fourier transform infrared spectroscopy (FT-IR), contact angle, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) measurements. The prepared membranes exhibited controllable temperature-dependent swelling behaviors and surface hydrophilicity which highly regulates the attachment of Gram-negative bacteria (E. coli). At a physiological temperature, the high content of the non-ionic polyNIPAAm in grafting copolymeric layer exhibits a high bacterial attachment due to the interfacial exposure of polyNIPAAm-rich hydrophobic domains. A relatively high content of polySBMA in grafting copolymeric layer exhibits reduced amounts of bacterial attachment due to the interfacial hydration of polySBMA-rich hydrophilic segments. This work shows that the bioadhesive properties of CA membranes with bi-layer copolymer grafting can be effectively controlled to be potentially useful for triggered bacteria detachment. The tunable-bioadhesive behavior of temperature-sensitive
PNIPAAm-SBMA grafted CA membranes makes this smart membrane appropriate for biomedical applications.

並列關鍵字

anti-biofouling ； plasma treatment ； cellulose acetate ； Stimuli-responsive membrane ； LCST

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