抗氧化劑之血液透析動力學模式與試驗探討

Method for Antioxidant Delivery from Dialysate in Hemodialysis

吳妙玲(Miao-Ling Wu)

指導教授 ： 張炎林

中原大學/工學院/生物醫學工程研究所/碩士(2010年)

https://doi.org/10.6840/cycu201001052

摘要

腎衰竭病人靠血液透析移除血中的尿毒素。血液透析治療時，病人血液接觸到生物不相容的人工透析膜而引發免疫系統活化導致體內氧化壓力上升。目前一般都用口服抗氧化物質或改用生物相容性高的透析膜的膜材來降低血中的氧化壓力，但效果皆有限。
本實驗分為兩階段，一為先從具有抗氧化能力的植物萃取物如綠茶萃取物-兒茶素、單一綠茶萃取物-EGCG、紅景天、葡萄籽及印地安鵝苺。用不同的抗氧化能力分析方法包括多酚類含量測試、總抗氧化能力(FRAP)、過氧化氫清除能力及超氧陰離子清除能力等，篩選出最佳抗氧化萃取物為EGCG。二為將最佳抗氧化萃取物EGCG，直接溶於血液透析用之透析液中，依照其安全濃度調配成具”抗氧化能力的透析液”，實驗發現抗氧化透析液在經過30分鐘後至300分鐘內皆能持續供給定量的EGCG，在血液透析過程中其透析液皆具有抗氧化能力與清除DPPH能力。因此病人在做血液透析治療同時，透析液中的抗氧化成份即能直接清除血中產生的氧化物質，有效降低因氧化壓力所產生相關併發症之機率，提升血液透析病人之透析治療品質。

關鍵字

抗氧化能力 ； 血液透析 ； 透析液 ； 氧化壓力 ； 腎衰竭

並列摘要

The removal of uremic toxins in the patients with end-stage renal failure depends on hemodialysis. In hemodialysis, the blood of patients exposed to bioincompatible dialysis membranes caused activation of the immune system leading to increased oxidative stress. In general, the oral supplementation of antioxidants is capable or biocompatibility of dialysis membranes with high membrane to reduce blood levels of oxidative stress, but the results are finite. The purpose of this study is focusing on the development of dialysate with antioxidant capability.
The experiment was divided into two stages. First, plant extracts with antioxidant functions include green tea extract, EGCG, Rhodiola rosea , Grape Seed and AMLA, the use of different antioxidant capacity analysis include total phenolic content , total antioxidant capacity by FRAP(Ferric Reducing Ability of Plasma), hydrogen peroxide scavenging and superoxide anion scavenging ability, and selected the best antioxidant extracts for EGCG.
Second, as the best antioxidant extract EGCG, directly soluble in blood dialysis concentration A , as a "antioxidant of dialysis solution," antioxidant dialysis solution was found after 30 minutes until 300 minutes a sustained supply of quantitative EGCG, in the course of the hemodialysis dialysate its considerable antioxidant capacity and scavenging DPPH, so patients do hemodialysis at the same time, dialysis fluid antioxidants that can clear the blood produced by the direct oxidation of substances produced by reducing oxidative stress due to complications related to the probability of hemodialysis patients improve the quality of dialysis treatment.

並列關鍵字

Oxidative stress ； Renal failure ； Antioxidant capability ； Dialysate ； Hemodialysis

參考文獻

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