智能型pH感應性之雙離子水膠薄膜製備及其生物相容性質探討

Preparation of intelligent pH-responsive zwitterionic soft membranes and its bioadhesive properties

黃俊瑋(Chun-Wei Huang)

指導教授 ： 張雍

中原大學/工學院/化學工程研究所/碩士(2011年)

https://doi.org/10.6840/cycu201100524

摘要

中文摘要
本研究製備可調控生物分子於材料表面沾黏情形的功能型水膠薄膜，由具有pH感應性且帶正電荷組成的2-(N,N-dimethyl amino)ethyl Methacryate(DMAEMA) 和帶有雙離子組成的Sulfobetaine Methacrylate (SBMA)，經由調控正電荷組成和雙離子組成的莫耳比例可精準製備出不同莫耳比例組成的poly(DMAEMA-co-SBMA)水膠薄膜，並可調控生物分子於材料表面沾黏情形；例如:血漿蛋白吸附、血小板或細胞貼附以及細菌的吸脫附。本研究所製備之水膠薄膜在酸性(H+)或鹼性(OH-)環境中顯示出高度的pH 感應特性來可控制水膠薄膜之膨潤度。材料表面與生物分子和菌體之間的作用力，可經由蛋白質吸附、細胞貼附和細菌貼附行為證明，使我們了解生物分子貼附之性質。本研究使用酵素免疫連結反應(ELISA)測試水膠薄膜對於三種單一血漿蛋白及100%人體血漿蛋白吸附在表面的情形。當材料表面正電荷組成(DMAEMA)含量較多時，可觀察到會引起血小板的貼附和細胞的貼附情形發生。當添加了雙離子組成(SBMA)後，會加速水層的形成，結果顯示能加強的使細菌在材料表面上產生脫附現象。這種具有良好的生物相容性，可應用pH值來調控生物分子於材料表面沾黏程度，未來可廣泛的應用在生醫方面。

關鍵字

可調控生物沾黏性質表面 ； pH感應性 ； 水膠 ； 觸發細菌脫附

並列摘要

Abstract
This work describes a novel tunable-bioadhesive soft membrane of pH-responsive 2-(N,N-dimethyl amino)ethyl methacrylate (DMAEMA) containing zwitterionic sulfobetaine methacrylate (SBMA). This novel soft membrane highly regulates general bioadhesive foulants through the adsorption of plasma proteins, the adhesion of human platelets and cells, and the attachment of bacterial. The copolymeric soft membranes exhibited controllable pH-dependent swelling behaviors and showed stimuli-responsive phase characteristics in the presence of cationic ions (H+ )or anionic ions (OH-). The interactions of these soft membranes with biomolecules and microorganisms were demonstrated by protein adsorption, cell adhesion, and bacterial attachment, which allowed us to evaluate their bioadhesive properties. An enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies was used to measure different plasma protein adsorptions on the prepared soft membrane surfaces. The attachment of platelets and the spreading of cells were only observed on polyDMAEMA-rich soft membrane surfaces. Interestingly, the incorporation of zwitterionic SBMA units into the polyDMAEMA soft membranes was found to accelerate the hydration of the bacterial attached surfaces and resulted in more rapid bacterial detachment. Such copolymer gel surface was shown to be potentially useful for triggered bacterial detachment. This work shows that the bioadhesive properties of poly(DMAEMA-co-SBMA) hydrogels can be effectively controlled via regulated ionic and zwitterionic molar mass ratios. The tunable-bioadhesive behavior of pH-sensitive poly(DMAEMA-co-SBMA) makes this biocompatible hydrogels appropriate for biomedical applications.

並列關鍵字

Tunable-bioadhesive surface ； pH-sensitive ； Hydrogels ； Triggered bacterial detachment

參考文獻

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