直接合成及鑑定具有機螢光物質奈米尺寸中孔洞材料

Direct synthesis and characterization of mesoporous silica nanoparticle with fluorescence dye

何右正(Yu-Cheng He)

指導教授 ： 鄭吉豐

中原大學/理學院/化學研究所/碩士(2011年)

https://doi.org/10.6840/cycu201101064

摘要

本論文以生物細胞之標定為研究目標，選用含螢光染料之奈米孔洞材料作為研究之對象，進行研究。
一般合成含染料之奈米孔洞材料，以染料作為起始物進行改質、修飾。將改質後染料鍵結於修飾過後之奈米孔洞材料上。本論文將採用不同方式合成含染料之奈米孔洞材料，利用Friedel-Crafts反應於奈米孔洞材料表面上固相合成及鍵結所需之染料。
本論文將使用兩種二氧化矽奈米孔洞材料作為後續研究之基材。一為奈米尺寸孔洞材料MSN(Mesoporous Silica Nanoparticle)，孔洞及顆粒大小約為2.6 nm及70 nm;另一為SBA-15奈米中孔洞分子篩，孔洞及顆粒大小約為10 nm及400 nm。利用其高表面積與孔洞性質，使染料之鍵結量提高，孔洞性也能使染料對外界環境更加敏感。
本論文選用三種生物標定螢光物質，第一種是F，這是一般常見用於生物標定之染料，它會發出特定之綠色螢光。第二種染料為B，會發橘黃色螢光。第三種G會發黃色螢光，可用作染料和染料激光增益介質。
以具有高度生物相容性之二氧化矽奈米孔洞材料作為基材，合成具有螢光性質之奈米孔洞材料。此合成方法不但稀有，不需使用含特殊官能基之昂貴染料，也可任意調控反應起始物，合成所需之染料。在生物細胞標定之應用上也具實際效果。

關鍵字

中孔洞 ； 奈米尺寸 ； 螢光染料

並列摘要

Nano-sized nanoporous materials tethering carboxylic indicators were prepared via direct-synthesis and co-condensation. Indicators with carboxylic group were synthesized on nanoporous materials via Friedel-Crafts reaction. These nanoporous materials with indicators can be used for trace of biological cells.
Two kinds of nanosized nanoporous material were synthesized. One was MSN (mesoporous silica nanoparticle) with porosity of 2.6 nm and particle size of 70 nm. Another was SBA-15NPs (SBA-15 nanoparticles) having 10 nm porous and 400 nm particle size. Indicators on MSN and SBA-15NPs were bonded after synthesis.
Three kinds of bonded indicators were chosen and studied because of their unique properties. The three bonded indicators emitted green fluorescence and orange fluorescence. Their characteristic fluorescence intensity variation with various pH values in solution were monitored by photography and fluorescence spectroscopy. MSNs with indicators were effectively endocytos into cells as a cell markers.
Synthesized nanoporous materials with indicators via Friedel-Crafts reaction are inexpensive, simple and rare as compared to commercially available dyes. Synthesis of different indicators can be achieved by simply adding different reactants. In addition, Silica as the component of the synthesized nanomaterial has high biocompatibility and stability. Thus, mesoporous nanoparticles with indicators can be applied not only as a cell maker but also a medicine carrier.

並列關鍵字

fluorescence dye ； nanoparticle ； mesoporous

參考文獻

1.McBain, J. W. In The Sorption of Gases and Vapours by Solids: Rutledge and Sons, London, 1932 ; ch. 5.

2.IUPAC Manual of Symbols and Terminology, Appendix 2, Part 1,

S. Nature, 1992, 359, 710.

7.Lin, Y. C.; Chen, S. H.; Itri, R. J. Phys. : Condens. Matels 1996, 8, 12169.

9.Zuna, R. Colloids Surf. 1997, 27, 123.