- 學位論文
探討垃圾滲出水處理廠之厭氧氨氧化菌族群分布
Investigation of the Distribution of Anaerobic Ammonia-Oxidation Bacteria in Landfill-leachate Treatment Plant
摘要
一般傳統生物處氮程序，處理程序繁複且需佔用大量的土地體積與額外添加碳源的缺點，若利最為節能的厭氧氨氧化(ANAMMOX)生物處理程序較傳統生物硝化脫硝技術可節省60％以上的費用，是一種不需耗費大量能源與無毒性的處理程序。 因此採用厭氧氨氧化(ANAMMOX)生物處理程序的垃圾廢水處理廠作為探討研究對象，此處理廠所處理垃圾滲出水的操作程序為SNAD程序是由序批次式反應器(SBR)衍生出來，SNAD程序結合部分硝化、厭氧氨氧化及異營性脫硝三種作用於同一反應系統中，氨氮與COD可同時被去除在同一個反應槽。 本研究將樣品分為兩種，一為紅色顆粒(G1、G2、G3)；二為混合紅色顆粒和污泥之混合樣品(M1)。針對真細菌(EB)、氨氮氧化菌（AOB）、亞硝酸氧化菌（NOB）、脫硝菌、總厭氧氨氧化菌(TA)、Brocadia anammoxidans (BA)、Kuenenia stuttgartiensis (KS) 利用分子生物技術進行研究探討。 其相對定量結果顯示G1與M1樣品於紅色顆粒比例不同導致厭氧氨氧化菌的含量差異很大，故紅色顆粒含量多寡，可提供實廠操作營運好壞之參考。 絕對定量分析G1、M1、G2發現具有共同特性為Kuenenia stuttgartiensis在樣品中的含量遠高於 Brocadia anammoxidans。 利用Brod541F與Amx820R、 16S-5’與16S-3’兩對引子，針對厭氧氨氧化菌進行選殖，藉此選擇最適合做為此研究的引子；最後利用11F與1512R作為16S rRNA 真細菌分析，發現大多數為脫硝菌種，並試圖找尋硝化菌。經由選殖技術以了解紅色顆粒內的菌相生態系，找尋台灣垃圾滲出水的厭氧氨氧化菌優勢菌種。
並列摘要
The traditional process of biological nitrogen removal has some disadvantages, including high complexity, occupation of large land area, and dependence on additional carbon source. However, if anaerobic ammonia-oxidation (ANAMMOX) were used, the cost of ANAMMOX than nitrification and denitrification technology could decrease by 60% up. the ANNAMOX process don’t need to spend a lot of energy and is non-toxic. . Therefore, studying the ANAMMOX bacteria is the leachate from landfill treatment plants, in particular the plants that operate using the SNAD process that is derived from the sequence batch reactor (SBR) ,SNAD combines partial nitrification, anaerobic ammonium oxidation, and heterotrophic denitrification in the same reaction system to remove ammonia and COD simultaneously. In this study, the sample is divided into two types: pure red granule (G1, G2, and G3), and a mixture of red granule and sludge (M1). Biotechnological techniques were used to study eubacteria (EB), ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), denitrifying bacteria, total anaerobic ammonium oxidizing bacteria (TA), Brocadia anammoxidans (BA), and Kuenenia stuttgartiensis (KS). The relative quantification results show that red granules (samples G1 and M1) affect the amount of aerobic ammonia oxidation bacteria. Hence, the granule content was used as the reference of the landfill-leachate treatment plant operating condition. Absolute quantification analysis shows that the amount of Kuenenia stuttgartiensis was higher than Brocadia anammoxidans (samples G1, G2 and M1). Cloning was used to assess the ecosystem within the red granule. Universal primers 11F and 1512R were used to clone 16S rRNA of eubacteria. And then, two primers Brod541F and Amx820R and 16S-5’ and 16S-3’ were evaluated to select the most suitable primer for cloning ANNAMOX-utilizing bacteria. We found that most are denitrifying bacteria. Further studies are being done to find nitrifying bacteria. Finally, we look for the dominant strain of ANAMMOX bacteria on landfill-leachate treatment plant in Taiwan.
參考文獻
延伸閱讀
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