電位處理技術於葡萄糖感測電極之去汙探討

The de-biofouling studies of using potential treatment technology for glucose sensing electrodes

趙書瑤(Shu-Yao ,Zhao)

指導教授 ： 婁世亮

中原大學/工學院/生物醫學工程研究所/碩士(2012年)

https://doi.org/10.6840/cycu201200793

摘要

植入式感測器植入體內所面臨最大困難為生物汙化，量測訊號隨之降低而導致感測器失效，本研究之目的為應用電位處理去汙技術於植入式葡萄糖生物感測電極，克服生物汙化之情形，進而提供感測電極長時間量測訊號穩定性，達到植入式葡萄糖感測電極之功能。運用本實驗室已開發之三極式微型感測電極，完成製備葡萄糖酵素感測電極，經由掃描式電子顯微鏡觀察得表面保護膜厚度為9.76 ± 5.38m，並得此微型葡萄糖感測電極之靈敏度為0.078 A/mM，線性迴歸係數為0.966，於葡萄糖濃度範圍0~5 mM有良好的線性度。置於細胞間質替代液2小時模擬體內生物汙化之情形，，存留訊號反應百分比為17.47 ~ 39.54 %。進一步在體外模擬環境下進行電位去汙處理技術，施加負電位於工作電極表面並根據電荷同性相斥之物理特性，給予貼附於電極表面之蛋白質剪應力使蛋白質剝除以達成去汙化效果。電位去汙處理技術應用於微型葡萄糖感測電極之最佳處理參數為交互電位，電位大小-1 V與0 V，時間18秒，電位變換頻率為500 Hz，訊號百分比恢復量為4.65 ~ 30.44 %。並透過去汙化技術重複可行性之驗證，證實電位去汙處理技術為長久可行性之去汙方法。總言之，本研究由實驗結果得知電位去汙處理技術應用於植入式葡萄糖生物感測電極之效果與完成可行性評估。

關鍵字

電極 ； 植入式 ； 去汙化技術 ； 葡萄糖

並列摘要

Biofouling on implantable biosensor results in the sensitivity reduction and sensor failure during implantation is the most important issue for consideration. Biofouling is a consequence of inflammatory, which produces the accumulation of proteins, cells and other tissues on the surface of foreign sensors. The goal of this study is to diminish biofouling on electrodes of glucose sensors applying electric potential technology. The electrodes of tri-poles miniature sensor for glucose detection, which were applied in this study, had had been developed previously. After improvement, the protective film thickness and sensitivity of the electrode surface are 9.76 ± 5.38 m and 0.078 A/mM individually. The linear regression is 0.966 during the glucose concentration 0 to 5 mM in PBS. The residual signal response is 17.47 % to 39.54 %. Furthermore, the negative voltage was exerted on the working electrides for simulating the potential treatment in vitro. Results showed that the proteins were detached from the surface of electrodes due to the electrostatic principle. Alternative potential at -1 V and 0 V were appropriate for de-biofouling during 18 seconds treatment. The alternative frequency was 500 Hz and the signal recovery is 4.65 % to 30.44 %. Therefore, the potential treatment was an effective method for long-term de-biofouling has been approved. In conclusion the assessment and efficacy of an innovative method for de-fouling, the potential treatment had had been accomplished. It could be applied on implantable glucose sensors to solve the biofouling in the future.

並列關鍵字

Biofouling ； De-biofouling ； Glucose sensing electrodes ； Implantable

參考文獻

20. 蔡瀚慶，植入式葡萄糖感測電極於細胞間質替代液之特性探討，中原大學生物醫學工程研究所，碩士論文，2010。

21. 楊泱澧，植入式葡萄糖感測器之設計製作與動物實驗探討，中原大學生物醫學工程研究所，碩士論文，2011。

22. 林建廷，植入型葡萄糖感測系統之動物實驗探討，中原大學生物醫學工程研究所，碩士論文，2011。

1. The Diabetes and Nutrition Study Group of the Spanish Diabetes Association. Diabetes Nutrition and Complications Trial: adherence to the ADA nutritional recommendations, targets of metabolic control, and onset of diabetes complications. A 7-year, prospective, population-based, observational multicenter study. J Diabetes Complications 2006;20:361-366.

3. Rathmann W, Giani G. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:2568-9; author reply 2569.