螢光金奈米團簇與白蛋白形成暈狀體之螢光能量共振轉移分析

Analysis of Gold Nanoclusters-Protein Bioconjugate (Corona) Self-assembly by Fluorescent Resonance Energy Transfer

陳卿達(Ching-Ta Chen)

指導教授 ： 林政鞍 ； 張恒雄

中原大學/工學院/生物醫學工程研究所/碩士(2012年)

https://doi.org/10.6840/cycu201201006

摘要

螢光金奈米團簇具有獨特發光特性、生物相容性較佳且具有相當高表面積比率的特性，可做為新一代螢光探針開發並且應用在臨床醫學上，故探討螢光金奈米團簇與血漿蛋白之間的交互作用是重要研究課題。本研究中選用自製二氫硫辛酸批覆之金奈米團簇，與血漿中含量最豐富之白蛋白，探討靜電力吸附後相關性質之改變。結果發現奈米團簇金與蛋白吸附後會產生三種吸附結構，主要是利用白蛋白上離氨酸側基與金奈米團簇促成靜電力吸附，此自組裝結構不僅提升螢光金團簇之發光強度，並進一步影響其細胞攝入行為。本研究提供超微奈米材料與生物蛋白之交互作用，對於奈米醫學生醫應用發展提供更多的基礎評估。

關鍵字

白蛋白 ； 二氫硫辛酸 ； 奈米金團簇 ； 離氨酸

並列摘要

Fluorescent gold nanoclusters has shown unique luminescent properties, good biocompatibility and high surface ratio which can be a new generation of fluorescent probes and applied in clinical medicine. Therefore, to explore the interaction between fluorescent gold nanoclusters with plasma protein is an important topic. In this study, the dihydrolipoic acid-protected gold nanoclusters, and the serum albumin were explored the change of properties after assembling via electrostatic force. Three structures can be formed after gold nanoclusters adsorbing onto plasma protein. Lysine-rich domain of albumin may offer assembling force with gold nanoclusters electrostatically. These self-assembled structures not only enhance the fluorescent intensity of gold nanoclusters, but affecte the behavior of cellular uptake. In this study, the interaction between micro/nano materials and protein can provide the basis of assessment for the nanomedicine biomedical applications development.

並列關鍵字

lysine ； gold nanoclusters ； dihydrolipoic acid ； serum albumins

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