龍眼花萃出物調節血糖功效之研究

Effect of Longan Flower Extract on Blood Glucose Regulation

王詠萱(Yung-Hsuan Wang)

指導教授 ： 吳亮宜

中原大學/工學院/生物科技研究所/碩士(2012年)

https://doi.org/10.6840/cycu201201065

摘要

糖尿病是一種病患長期處於高血糖狀態的慢性疾病，為現代普遍疾病之一，不僅名列國人十大死因中，且盛行率逐年增加，病患年齡層也逐年下降。除此之外，糖尿病還會引發許多併發症的產生，例如心血管疾病、腎臟病、高血壓，可見糖尿病對於國人健康之重要程度。依據前人的研究結果顯示，龍眼花水萃物具有改善代謝症候群諸多症狀之功效。而代謝症候群經常出現在糖尿病患者身上，因此本研究之目的為評估龍眼花是否具有改善糖尿病症狀的功效，同時也評估其是否具有改善糖尿病併發症之潛力。
本論文首先運用Sprague-Dawley大鼠以Nicotinamide及Streptozotocin誘發第二型糖尿病後分別餵食龍眼花水萃物及其乙酸已酯層、水層區分物來評估龍眼花水萃物及區分層對於糖尿病各項症狀之改善情形。接著將龍眼花萃出物及各區分物進行葡萄糖及二羰基化合物誘發高階醣化終產物生成實驗來評估龍眼花抑制糖尿病併發症發生之可能潛力。
在動物實驗部分，結果顯示龍眼花水萃物乙酸乙酯區分層對於糖尿病的高血糖以及高三酸甘油酯症狀有改善效果。對於葡萄糖耐受性不良，龍眼花水萃物以及其乙酸乙酯層也有明顯改善的現象。在葡萄糖及二羰基化合物誘發高階醣化終產物生成研究部分則顯示龍眼花水萃物具有顯著改善高階糖化終產物生成的能力。而在龍眼花水萃物區分層中又以乙酸乙酯區分物的效果最為顯著。

關鍵字

血糖 ； 龍眼花

並列摘要

Diabetes mellitus (DM) is a chronic disease characterized by elevated levels of sugar in the blood. It's one of the prevalent diseases of modern times. DM not only list on the "top ten causes of death" in Taiwan, but also the prevelence rate of it increasing, accompanied with a decreasing average age of incidence annually. In addition, DM would also induce other complication diseases, for example coronary heart disease, renal disease and hypertention. Apparently, DM is a significant disease for people in Taiwan. Our previous study showed that water extract of longan flower (LF) could ameliorate the multiple symptoms in metabolic syndrome (MS). MS frequently occurs in people with DM. Therefore, the aim of this study was to further examine if the longan flower could improve DM and the complication of DM.
The first part of this study is an animal study. Nicotinamide and streptozotocin-induced type 2 diabetic Sprague-Dawley rats were used as a animal model. Rats were administered with water extracts of longan flower (LF), ethyl acetate fractions of LF (EA) and water layer fractions of LF (W) respectly to evaluate the hypoglycemic effect of LF and its different fractions. Following, the assay of glucose/dicarbonyl compound induced formation of advanced glycation end products were used to assess the preventing effects of LF and its different fractions on diabetic complication.
Results of in vivo study showed that EA fraction could improve the hyperglycemia and hypertriglyceridemia in diabetes rats. And also, LF and EA fraction ameliorated the glucose intolerance which is considered to be a stage in the development of type 2 DM. Results of in vitro study showed that LF could inhibit the formation of advanced glycation end product, and the most effective fraction was EA fraction.

並列關鍵字

Blood Glucose ； Longan Flower

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