由點擊離子配對控制帶正電性殺菌刷狀高分子於細菌可逆釋放之研究

Reversibly releasing bacteria from antimicrobial polycation brushes by clicking counterions

陳彥盛(Yen-Sheng Chen)

指導教授 ： 黃俊仁 ； 張雍

中原大學/工學院/化學工程研究所/碩士(2014年)

https://doi.org/10.6840/cycu201400910

摘要

由於一般正電性殺菌材料，因其表面電性和細菌表面電性相異，故使用一段時間後，死亡之細菌將使貼附於正電性殺菌材料表面，使其失去殺菌效果，所以改善此缺點是目前必須要探討的課題之一。
本研究以鹽類溶液對於四級胺官能基表面相互影響之作用力，使貼附於正電性殺菌材料表面死亡之細菌有效、迅速地進行脫附，並以離子置換的方式，將其表面亦可復原再生為可殺菌之四級胺官能基表面。
以表面接觸角量測儀(Contact angle, CA)，X射線光電子能譜儀(X-ray Photoeletron Spectroscope,XPS)，表面電位分析儀(Zeta potential analyzer)，橢圓偏光儀 (Ellipsometry) 進行物理、化學分析，觀察鹽類溶液對於四級胺官能基表面相互影響前後其表面物理、化學性質。並以大腸桿菌 (Escherichia coli) 、表皮葡萄球菌 (Staphylococcus epidermidis) 、嗜麥芽寡糖單胞菌 (Stenotrophomonas maltophilia)之貼附、脫附行為來探討鹽類溶液對於四級胺官能基表面作用，最後以表面電漿共振感測儀(Surface Plasma Resonance) 測量蛋白質之貼附、脫附行為，並且探討鹽類溶液對於四級胺官能基表面作用是否有可以應用於非細菌之生物分子上。

關鍵字

細菌 ； 點擊離子配對 ； 可逆釋放 ； 正電性殺菌刷狀高分子

並列摘要

Since the electrical properties of the surface of the general bactericidal materials is different from the bacterial surface, the dead bacteria will attach to the surface of the bactericidal materials, leading to a loss of bactericidal efficacy after a period of time. As a result, it is a great challenge to overcome this disadvantage.
In this study, we will release the dead bacteria which attached to the surface of the bactericidal materials by interacting the surface between quaternary amine groups and salt solution in a fast, efficiently, and reversibly fashion. And we will also regenerate the surface to kill bacteria again.
The physical and chemical analyses of the surface which interact between quaternary amine groups and salt solution were characterized by contact angle goniometer (CA), X-ray photoeletron spectroscope (XPS), zeta potential analyzer, and ellipsometry. The surface with quaternary amine groups allow attachment and releasing of Escherichia coli, Staphylococcus epidermidis and Stenotrophomonas maltophilia. Finally, the surface with quaternary amine groups for attachment and releasing of protein was measured by surface plasmon resonance.

並列關鍵字

clicking counterions ； reversibly release ； bacteria ； antimicrobial polycation brushes

參考文獻

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