金屬有機骨架材料之抗菌性與環境應用

ENVIRONMENTAL APPLICATIONS AND BACTERICIDAL PROPERTIES OF METAL ORGANIC FRAMEWORKS

羅安傑(Ankit Rawat)

指導教授 ： 黃郁慈

中原大學/工學院/生物環境工程研究所/碩士(2014年)

https://doi.org/10.6840/cycu201400916

摘要

金屬有機骨架（MOFs）材料因其多孔結構和可包覆金屬於結構中等特性，日漸在國際研究中引起討論，而成為新一代的抗菌化合物。細菌感染所引發的疾病或併發症，每年都會在世界各地造成大量病患的死亡，因此我們急需面對並處理此一威脅，研究高效穩定的抗菌劑。在這項研究中，我們評估MOFs材料的抗菌性能，即包覆MIL88B（鐵），MIL100（鉻），MIL100（Al）或MIL100（V）等，針對革蘭氏陰性菌大腸桿菌進行許多檢測，研究結果使用最低抑菌濃度（MIC）和半數最大抑制濃度（IC50）這兩個參數，作為這些材料抗菌活性的量度。在MIC之結果中，以鐵，鉻和鋁為核心要素的MOFs分別為5，40，6 mg/mL。以電子顯微鏡成像觀察材料與大腸桿菌之互動，也可使我們對於材料與細胞毒性的影響有進一步的了解。成像效果說明材料如何影響細菌細胞膜，破壞其形態特徵。經此一研究，我們將能有一個具高載藥量及生物可降解性等特徵之使用平台，提供生物醫學應用和MOFs材料的使用方針。

關鍵字

金屬有機骨架、抗菌、最低抑菌濃度、大腸桿菌、電子顯微鏡

並列摘要

Because of their highly porous structure and inclusion of a range metals, metal organic frameworks (MOFs) have drawn attention as next generation antimicrobial compounds. Bacterial infections cause a high number of fatalities each year worldwide. The severity of this threat motivated us to research highly efficient and stable antibacterial agents. In this study, we evaluated the antibacterial properties of MOFs namely, MIL 88B (Fe), MIL 100(Cr), MIL 100(Al) and MIL 100(V). The gram negative bacteria Escherichia coli served as the specimen for all assays. The minimum inhibitory concentration (MIC) and half maximal inhibitory concentration (IC50) were the two parameters used which gave us the measure of antibacterial activity. The MIC results for MOFs having Fe, Cr and Al as central element were 5, 40 and 6 mg/mL respectively. Scanning Electron Microscopy imaging was employed in an effort to advance our understanding of the cytotoxic impacts of the iron based MOF. The imaging results illustrated how the MOF adhere to bacterial cell envelope and disrupted its morphological features. Moreover, the images enabled identifying possible reasons for the bactericidal activity of MOFs. We describe a promising platform for biomedical applications based on the high drug loading capacity, biodegradability, and versatile functionality of MOFs.

並列關鍵字

Scanning-electron microscopy of E. coli. ； Minimum inhibitory concentration ； Metal-organic framework ； Antibacterial activity

參考文獻

[52] Lifeng Dong, 2 Michael M. Craig,3 Dongwoo Khang,4 and Chunying Chen5, Applications of Nanomaterials in Biology and Medicine, Journal of Nanotechnology, 2012 (2012) 2.

[1] F.J. Heiligtag, M. Niederberger, The fascinating world of nanoparticle research, Materials Today, 16 (2013) 262-271.

[2] M. De, P.S. Ghosh, V.M. Rotello, Applications of Nanoparticles in Biology, Advanced Materials, 20 (2008) 4225-4241.

[3] A. Kumari, S.K. Yadav, S.C. Yadav, Biodegradable polymeric nanoparticles based drug delivery systems, Colloids and Surfaces B: Biointerfaces, 75 (2010) 1-18.

[4] I. Brigger, C. Dubernet, P. Couvreur, Nanoparticles in cancer therapy and diagnosis, Advanced drug delivery reviews, 54 (2002) 631-651.