- 學位論文
實驗設計法分析連續式膜過濾技術植物性蛋白分離濃縮程序
Analysis of the continous membrane technology of the plant protein separation/concentration process by Experimental Design Methodology
摘要
非食用性且含油量高的麻瘋樹可用來製備生質柴油，製備過程會產生大量蛋白性廢棄物。將此廢棄物用來製造高價值產品(例如:天然性肥料以及木材黏著劑)，有助於整體生質能的發展。現今使用冷凍乾燥作為蛋白濃縮技術卻存在著耗能問題，因此本研究透過膜過濾 濃縮技術改善此問題。 本研究係以連續式膜過濾技術進行麻瘋蛋白濃縮程序，並結合 回應曲面法探討各操作因子對目標函數的影響。本研究討論濾膜孔徑大小、進料酸鹼值以及跨膜壓差間相互影響關係，以了解連續式膜過濾蛋白濃縮程序的滲透通量與蛋白阻擋率變化。 選用0.8 um膜孔徑濾膜進行3小時膜過濾程序，操作環境控制在麻瘋蛋白等電點(pH 5)及維持在0.75kg/cm2跨膜壓差，可得到80%蛋白阻擋率及滲透通量可維持在40 L/m2-h。最後將膜過濾濃縮與 冷凍乾燥技術進行比較。當濾膜面積放大至0.25 m2以上時，膜過濾濃縮技術與冷凍乾燥技術結合將以節能且省時方式達完成麻瘋蛋白濃縮程序並可以用於改善現有的其他蛋白濃縮程序。
並列摘要
Jatropha curcas has become a popular application in biodiesel process technology because of its non-edible crop and rich-oiled. The researches indicated that Jatropha residue exhibited ample protein, and could be applied in high value product (eg. natural fertilizer and wood adhesive). Fouling, larger membrane areas and frequent membrane cleaning is the main reason for increasing the energy consumption and operation cost. Therefore, the research is focus on establishing the math models to modify the shortness of membrane filtration and compares with the recently protein concentration technology. In this study, the optimized operation factor of protein concentration with Response surface methodology (RSM) such as membrane pore size(x1), pH value(x2) and transmembrane pressure(x3) were discussed the continuous membrane filtration technology of Jatropha protein concentration. The math models were built by RSM and the results showed that R^2 value for models were greater than 0.9. The optimal result of protein rejection is above 80% and flux with 40 L/m2-h in the operation factor (x1= 0.8um, x2= 5 and x3= 0.75 kg/cm2). While the area of membrane is more than 0.25 m2, the result is more energy efficiency and saving-time with freeze-drying. In that, the research indicates the optimal factors to improve the efficiency protein and water separation with membrane technology.