奈米銀粒子對小鼠早期胚胎發育的影響

奈米銀粒子 (Ag-NPs) 在許多抗菌產品中作為一種抗菌成份。先前的研究發現奈米銀對於小鼠囊胚具有細胞毒性且會造成發育上的缺陷。然而我們對於奈米銀在早期胚胎的副作用仍然不清楚。在此研究中欲探討奈米銀粒子對小鼠早期胚胎的胚胎毒性。我們先將小鼠的早期胚胎分別共培養濃度為3、6及12μM的奈米銀粒子，並利用Hoechst 染色、TUNEL分析法、體外發育及DCF-DA染色去觀察細胞增生、細胞凋亡、早期胚胎體外發育及細胞內活性氧物質等的變化。從結果發現6及12μM奈米銀粒子會影響小鼠早期胚胎的型態，並造成胚胎發育至囊胚的比例明顯下降。此外，在6μM奈米銀處理後會使囊胚的細胞增生數減少並造成細胞凋亡的數量增加。而在3μM奈米銀處理後即會抑制胚胎體外發育的能力。早期胚胎與奈米銀共培養會使細胞內的活性氧物質增加，而加入NAC後能夠抑制奈米銀所誘發的活性氧物質。此研究結果顯示奈米銀可能會誘發小鼠胚胎產生細胞毒性。奈米銀誘發的細胞毒性可能與活性氧物質產生有關。

關鍵字

細胞凋亡；奈米銀；胚胎

並列摘要

Silver nanoparticles (Ag-NPs) have been widely used as an antimicrobial coating in various antibacterial products. Previous studies found a cytotoxic effect of Ag-NPs on mouse blastocysts and its association with defects in subsequent development. However, the biological side effect on early embryos development remains unknown. In the present study, we investigate the embryo cytotoxicity of Ag-NPs on mouse early stage embryos. The mouse early stage embryos were co-cultured with 3, 6 and 12 μM Ag-NPs. Cell proliferation, apoptosis detection, early embryonic outgrowth and generation of reaction oxygen species (ROS) were evaluated by Hoechst staining, TUNEL assay, embryo transfer and DCF-DA staining, respectively. Early stage embryos were treated with 6 and 12 μM Ag-NPs exhibited significantly damage morphology. Percentage of embryo development to blastocyst stage was decreased at treatment concentration higher than 6 μM Ag-NPs. The results also showed that Ag-NPs (6μM) inhibited cell proliferation and significantly induced cell apoptosis in blastocyst stage embryos. Moreover, Ag-NPs inhibited early embryonic outgrowth at 3 μM. Intracellular ROS level was also increased in Ag-NPs-treated early stage embryos. NAC pretreatment could effectively prevent Ag-NPs-induced ROS production. Taken together, the study results reveal that Ag-NP has the potential to induce mouse embryo cytotoxicity. Ag-NPs induced cytotoxicity maybe related to ROS generation.

並列關鍵字

silver nanoparticles ； apoptosis ； embryo

參考文獻

Ahamed, M., R. Posgai, T.J. Gorey, M. Nielsen, S.M. Hussain, and J.J. Rowe. 2010. Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicology and applied pharmacology. 242:263-269.

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奈米銀粒子對小鼠早期胚胎發育的影響

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