摘要
我們建構光譜造影系統觀察螢光標定的生物細胞，此系統以倒立式螢光顯微鏡為基礎並可執行廣域造影和掃描式螢光造影。系統中，倒立式顯微鏡的平台上整合活體細胞培養箱，此活體細胞培養箱能調控系統濕度及溫度和氣體含量，因此細胞能置於培養箱中長時間培養並能進行縮時造影。此外，我們提出演算法和自動化程式，能加強影像對比和移除背景雜訊，經由影像處理後，影像的對比度提高，並可以明顯看到生物細胞的細微結構被凸顯出來。我們對掃描式造影系統進行測試，其橫向解析度為5.52 μm。此造影系統能觀察多螢光標定的生物細胞，因此我們也提出以疊代為基礎的演算法，演算法能對螢光標記的生物細胞進行定量分析。
並列摘要
We constructed a spectral imaging system for observation of fluorescent labeled biological cells. The system is based on an inverted fluorescence microscope and can perform both of wide-field imaging and scanning fluorescent imaging. In the system, biological cells were cultured in a temperature- and humidity-controlled incubator which was integrated in the microscope such that the system was able to perform the observation with time-lapsed imaging in a long period. Furthermore, we proposed an algorithm and an automatically processed program for contrast enhancement and background removing of images. After image processing, the contrast of images was significantly enhanced and the fine structure of biological cells can be observed. The lateral resolution of the system was examined to be 5.52 μm. This system is capable to observe biological cells labeled with multiple fluorescent markers. We also proposed an algorithm based on the principle of iteration such that the distribution of fluorescent markers can be analyzed quantitatively.