應用核磁共振技術鑑定聚己內酯-聚乙二醇雙性共聚物及其末端接磷酸膽鹼的可能性探討

NMR-Based Technology for the Identification of Poly (ethylene glycol)-block-Poly(e-caprolactone) and the Investigation of which Grafting with Phosphorylcholine

方昭明(Chao-Ming Fang)

指導教授 ： 賈緒威

中原大學/理學院/化學研究所/碩士(2008年)

https://doi.org/10.6840/cycu200900202

摘要

雙基團的兩性共聚化合物因為具有良好的生物相容性與生物降解性，再加上其在水溶液中疏水端位於核心處而親水端位於外圍的特殊性，因此在生物醫學方面常作為包覆藥物的載體之用。
磷酸膽鹼 (PC) 分子基於其優良的生物相容性與類似生物細胞膜的特性，其在生物醫學上的應用也愈來愈受重視。
有鑑於上述兩種分子的生物相關特性，近年來興起一股將兩者予以結合以期獲得更高傳遞藥物效率的高分子材料的研究。
本篇研究的主旨在於以核磁共振技術，來驗證聚己內酯-聚乙二醇雙性共聚物的 OH 末端是否可以成功接上 PC 以成為三基團的共聚化合物。在核磁共振的研究技術中，我們採用了一維氫、碳、磷譜的定性與定量相關研究、二維譜的 COSY、HMQC、HMBC、HSQC 的化學鍵結確認實驗、以及擴散實驗 (diffusion) 的應用來證實三基團的共聚化合物是否被成功的合成出。
本篇研究分成兩大部份：第一部份是運用核磁共振技術來證明聚己內酯-聚乙二醇雙性共聚物此雙基團化合物在合成上滿足我們在生物醫學上應用的需求；第二部份是去證實工研院合成出的聚己內酯-聚乙二醇雙性共聚物在其末端是否可成功的接上 PC 而成為三基團化合物。

關鍵字

聚己內酯-聚乙二醇雙性共聚物 ； 磷酸膽鹼

並列摘要

Amphiphilic diblock copolymer exists good biocompatible and biodegradible properities. Meanwhile,amphiphilic diblock copolymer in aqueous solutions.its core-shell structure is commonly formed with a hydrophobic core surrended by a hydrated hydrophilic shell. Based on these characteristics， desire to exploit the properities of block copolymer micelles in biomedical applications， namely the efficacious delivery of hydrophobic drugs sequestered within micellar cores， has drawn more attention in biocompatible and biodegradible amphiphiles.

The purpose of this study is to use Nuclear magnetic Resonance (NMR) techniques to identify each reaction intermediates during the reaction of the target tri-block copolymer， Poly(ethyleneglycol)-block-Poly(-Caprolactone)
-Phosphoryl Choline， by grafting Poly(ethyleneglycol)-block-Poly
(-Caprolactone) with Phosphorylcholine. The pulse sequences used here include one-dimensional 1H， 13C， 31P experiments and two-dimensional COSY，HMQC，HSQC，HMBC experiments. Upon request to identify different molecules in the same spectrum， bi-polar diffusion experiment will be used for the mixture analysis.

There are two consequential section in this study. Firstly， we used the mentioned NMR techniques to confirm the Di-block copolymer， Poly(ethyleneglycol)-block-Poly(-Caprolactone)，has been successfully synthesized and purified. Secondly， to check if the design of grafting procedure by ITRI (Inductrial Technology of Research Institute) is successful to make the Di-block copolymer to attach the end group Phosphorylcholine.

並列關鍵字

Poly (ethylene glycol)-block-Poly(e-caprolactone) ； Phosphorylcholine

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