微流體生物反應器中幾何效應對質傳現象影響之數值研究

Numerical study of the effect of aspect ratio on the mass transport in a 3D microchannel bioreactor

曹培成(Pei-Cheng Tsao)

指導教授 ： 鄧志浩

中原大學/工學院/土木工程學系/碩士(2009年)

https://doi.org/10.6840/cycu200900300

摘要

摘要

　　生物反應器環境對肝細胞合成與解毒功能有很大影響，其中最重要的培養液濃度需大於細胞最低消耗限度才能維持細胞增殖並降低死亡率。因此需要了解反應器底部濃度分佈情形，以有限元素軟體數值模擬反應器的幾何形狀對其底部培養液濃度分佈的影響。對反應器底部中間濃度而言，二維模擬可替代三維之模擬結果取決於反應器之高寬比。二維與三維模擬方式對反應器底部中間濃度分佈確有其差異，三維模擬分析中多了渠寬方向上的速度剖面較為真實，反應器底層中間與側邊濃度差異隨高寬比(α)越小而增大。α、Peclet number(Pe)、Michaelis-Menten常數(Km)越小或Damkohler number(Da)越大中間與側邊供細胞吸收的培養液養份濃度的有效長度差異越大，為細胞整體發展而言，應在α大小、反應器長度及初始流速取得平衡。

關鍵字

微渠道生物反應器 ； 高寬比 ； 質傳

並列摘要

Abstract

　　The bioreactor environment has the very tremendous influence on the synthesis and the detoxification function of hepatocytes, the most important concentration of the nutrient fluid must be more than the lowest limit which the cell consume to be able to maintain the cell multiplication and to cut the mortality.
　　Therefore needs to find out the base concentration distribution of the
reactor, by finite element software numerically simulates the influence on
the geometrical shape of the reactor to its base concentration distribution of the nutrient fluid.
　　Speaking of the middle base concentration of the reactor, the two-dimensional simulation may substitute the three dimensional simulation to be decided by the aspect ratio α ( the ratio of height to width) of the reactor. The two-dimensional and the three dimensional form really has its difference to the middle base concentration distribution of the reactor, in the three dimensional simulation analysis it gets the velocity profile in channel width direction has been more real, the middle and side base concentration difference of the reactor increased along with the smaller aspect ratio of height to width.
　　The effective length difference of the nutrient fluid absorbed by the
cell increased along with smaller α, Peclet number (Pe), Michaelis- Menten constant (Km) or bigger Damkohler number (Da), speaking of the cell integral development, it should obtains the balance in α size, reactor’s length and initial flow velocity.

並列關鍵字

aspect ratio ； mass transfer ； Microchannel bioreactor

參考文獻

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