螢光蛋白之光學性質及其於生醫造影之應用

Optical Properties of Fluorescent Proteins and Their Applications in Bioimaging

高嘉鄖(Chia-Yun Kao)

指導教授 ： 許怡仁

中原大學/理學院/應用物理研究所/碩士(2008年)

https://doi.org/10.6840/cycu200800273

摘要

近幾年來，外生螢光染劑已被廣泛的應用在生物影像技術之上。在各類螢光染劑中，綠螢光蛋白 (GFP) 因其本身具有高穩定性與高發光效率的優勢，因此，常被應用於非侵入性分子與細胞生物間的標定。同時，並可用來監控基因的表現、蛋白質分布以及蛋白質之間的交互作用。在本篇論文中，我們提出一套變溫螢光量測系統，用來量測在不同溫度下樣品螢光特性之改變，而後我們提出一套演算法來分析樣品於不同溫度下螢光分佈的情形，進而探討樣品之熱效應。 光同調斷層攝影術 (OCT) 的研究大多著重於系統的改良，一般可分為系統的解析力與成像速度的改良。在此，我們主要針對成像技術改良上提出一套全反射式快速掃描的光延遲線系統，用來取代傳統速度較慢的步進馬達。本系統最大的特色就是系統本身結構緊密、穩定、容易製造並在掃描的過程中可降低光損耗。我們將此光延遲系統應用於 OCT 中，當系統內的掃描鏡轉動 ± 9.6°時，經由計算可達到樣品的掃描深度約為 2.9 mm 。而後透過實驗來驗證系統的可行性。 未來我們希望能將此兩套系統進行整合，並利用 OCT 來量測材料內部物質受熱效應影響之變化，並針對不同樣品內部之光學性質進行探討。

關鍵字

光延遲線 ； 光同調斷層攝影術 ； 螢光蛋白

並列摘要

Recently, the exogenous fluorescent agent has been applied to bio-imaging techniques widely. In different fluorescent agents, the green fluorescent agent has high-stability and high-luminescence efficiency superior in itself, thus, it usually used to the lable of noninvasive
molecular and cell biology. It also use to monitor the gene phenomenon, rotein distribution, and protein interaction at the same time.
In the thesis, we offer a spectrum analyzing system to use to analysis the fluorescent property changing of the sample in different temperature, and then, we also offer a algorithm to analysis the phenomenon of the fluorescent spectrum distributions. Furthermore, we will treat the thermal effect of the samples.
The more research of optical coherence tomography (OCT) is mainly the systems improvement. One is the resolution of the systems, and the other is imaging speed improvement. In the thesis, we focus on improve the imaging speed and provide a real time all reflective optical delay line system to alternate the traditional stepper motor. The best distinguishing of this system is closed, stable, easily fabricated, and in scanning process, it can lower the light loss. We applied this system to the OCT, when the scanning mirror of the system tuned ± 9.6°, the scanning depth of the sample will arrived 2.9 mm by calculated. Then we will identify the system capability by the experiment.
In the future, we hope to combine with these two system, and use OCT to analyze the changing with thermal effect inside structure, and treat to inside optical properties.

並列關鍵字

optical coherence tomography ； fluorescent protein ； optical delay line

參考文獻

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