鎳鈷奈米線之紙基晶片應用於非酶葡萄糖檢測

Paper-based chip with nickel-cobalt nanowire on non-enzymatic glucose detection

葉芷綾(Chih-Ling Yeh)

指導教授 ： 張耀仁

中原大學/工學院/機械工程研究所/碩士(2018年)

https://doi.org/10.6840/cycu201800494

摘要

本論文提出將鎳鈷奈米線之紙基晶片應用於非酶葡萄糖電化學檢測上。使用陽極氧化鋁模板利用電沉積製備鎳鈷奈米線，並經由場發射掃描式電子顯微鏡表徵所製備之奈米線。另外，對於修飾網版印刷碳電極(SPCE)使用石墨烯作為奈米支架、不同線徑的鎳鈷奈米線影響與其所需之用量，透過循環伏安法探討葡萄糖的電催化效果，因而製得最佳優化之紙基晶片。基於實驗結果，滴加12 μl 之線徑200 μm 的鎳鈷合金奈米線於已使用石墨烯修飾之SPCE 紙基晶片上有最佳的催化效果。該紙基晶片能夠在葡萄糖濃度為4 μM~8 mM 的寬檢測範圍內檢測葡萄糖，這含蓋了生理水平範圍，其最低檢測極限為4 μM。另外，其也具有良好的抗干擾能力，甚至不受氯離子干擾。因此，此修飾之紙基晶片於非酶葡萄糖檢測中具有良好的檢測能力。

關鍵字

鎳鈷奈米線 ； 紙基晶片 ； 電催化 ； 非酶葡萄糖檢測

並列摘要

This thesis presents a paper-based chip with nickel-cobalt nanowires for non-enzymatic glucose electrochemical detection. Nickel-cobalt nanowires were prepared by electrodeposition using anodized aluminum template. The as-prepared nanowires were characterized by field-emission scanning electron microscope. In addition, the condition to modify the surface of screen printed carbon electrodes (SPCE) using graphene as a nano-framework for nanowires, the effect of different diameters of nanowires, and the dose of required nanowires were discussed to study the electrocatalytic effect for glucose by cyclic voltammetry. Thus, the optimized paper-based chip was obtained. Based on the experimental results, the paper-based chip with 12 μl of 200 μm Ni-Co nanowire dropcasted on graphene of SPCE had the best catalytic effect. This chip was able to detect glucose within a wide range of concentrations from 4 μM to 8 mM, which covers the range of physiological levels. The detection limit was 4 μM. Moreover, the chip had good anti-interference ability, and was not inferenced by chloride ions. Therefore, the the modified paper-based chip had a good detection ability in non-enzymatic glucose detection.

並列關鍵字

nickel-cobalt nanowire ； paper-based chip ； electrocatalysis ； non-enzymatic glucose detection

參考文獻

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