台灣藜外殼水萃出物改善代謝症候群功效之探討

Investigation of the Effect of Chenopodium formosanum Koidz. Husk Water Extract on Metabolic Syndrome

鄭翎筠(Ling-Yun Cheng)

指導教授 ： 吳亮宜

中原大學/理學院/生物科技研究所/碩士(2016年)

https://doi.org/10.6840/cycu201600905

摘要

隨著社會大眾生活型態與飲食習慣的改變，營養過剩及肥胖人口不斷增加，代謝症候群逐漸成為一個需要被關注的健康議題。且代謝症候群已被證實會增加第二型糖尿病及動脈粥樣硬化引起之心血管疾病的患病率。而現代人食用保健食品的觀念日益增進，且已有文獻指出台灣藜可能具有調節血糖之潛力。本實驗室先前的研究已證實短效性給予台灣藜外殼水萃物，可改善高果糖飲食所誘發之代謝症候群大鼠葡萄糖耐受性不良的情況。故本實驗欲探討台灣藜外殼水萃物是否具有改善代謝症候群之功能。
　　實驗設計將雄性 SD 大鼠分為四組，分別為餵飼一般飼料的基礎組 (B)、餵飼 60 % 高果糖飼料的控制組 (C)、餵飼 60 % 高果糖飼料中混入 0.5 % 台灣藜外殼水萃物之低劑量組 (L) 及餵飼 60 % 高果糖飼料中混入 1 % 台灣藜外殼水萃物之高劑量組 (H)。實驗為期 13 週。實驗期間測量體重、血壓及葡萄糖耐受性試驗 (OGTT)，並收集禁食血漿測量血漿葡萄糖、三酸甘油酯、總膽固醇、胰島素濃度等數值。實驗期結束收集檢體測定生化相關指標。
　　結果顯示 C 組之血壓、三酸甘油酯、膽固醇、肝臟三酸甘油酯及腹部脂肪皆顯著高於 B 組。而胰島素敏感性則顯著低於 B 組。相較於 C 組，餵飼台灣藜外殼水萃出物顯著改善高果糖飼料誘發之高血脂症、高血壓、胰島素阻抗及脂質堆積。同時在實驗中發現，台灣藜外殼水萃物藉由增加胰島素受體受質 1 及葡萄糖轉運蛋白 Ⅳ 的表現量及胰島素受體與 Akt 之磷酸化活性進而達到改善胰島素阻抗之效果。顯示台灣藜外殼水萃物能有效改善代謝症候群。

關鍵字

代謝症候群 ； 高果糖飲食 ； 台灣藜外殼 ； 胰島素阻抗

並列摘要

The metabolic syndrome (MS), which is cause by the wake of surplus energy intake, increasing obesity, and life habits, is a major and escalating challenge for public health. The metabolic syndrome includes central obesity, hyperglycemia, hypertension and dyslipidemia. Furthermore, many studies have shown that metabolic syndrome is an important risk factor for type 2 diabetes and cardiovascular diseases. Previous study has shown that Chenopodium formosanum Koidz. (CFK) could enhance the glucose uptake of adipocyte. Our previous study also found that short term CFK hush water extract manipulation improved the glucose intolerance in MS rats. Therefore, in this study we investigated the long term effect of the supplementation of CFK husk water extract on metabolic syndrome.
　　The male SD rats are divided into four group：basal group (B), fed with normal chow diet ; control group (C), fed with 60 % fructose diet ; low-dose group (L), fed with 60 % fructose diet plus 0.5 % CFK husk water extract ; high-dose group (H), fed with 60 % fructose diet plus 1 % CFK husk water extract. The study was last for 13 weeks. The blood pressure, fasting plasma glucose, insulin, triglyceride, total cholesterol and abdominal fat mass were measured. The oral glucose tolerance test (OGTT) was also performed in this study.
　　The data shown that the plasma triglyceride, total cholesterol, hepatic triglyceride, systolic blood pressure and abdominal fat mass were significantly increased in group C. Insulin sensitivity of rat in group C was lower than in group B. These data indicated that high fructose diet could cause a cluster of disorders in metabolic syndrome. CFK husk water extract supplementation significantly ameliorated hyperlipidemia, hypertension, insulin resistance (IR) and fat mass accumulation. We also found that supplementation of CFK husk water extract ameliorated IR by enhancing the protein expression content of IRS-1 and GLUT4, and the phosphorylation activity of insulin receptor and Akt. These findings indicated that Chenopodium formosanum Koidz. husk water extract supplementation is effective for improving metabolic syndrome.

並列關鍵字

metabolic syndrome ； high fructose diet ； Chenopodium formosanum Koidz. husk ； insulin resistance

參考文獻

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