- 學位論文
電透析技術在食品分析之樣品前處理的方法開發
Development of Electrodialysis Used As Sample Pretreatment of Food Analysis
摘要
摘要 在分析儀器中，因為其分離能力佳與高效率，因此毛細管電泳佔有舉足輕重的地位，不論在生化領域、食品添加物或環境檢測都有其相關應用。然而當分析物本身基質干擾嚴重，或是樣品中分析物濃度過低會使得在分析物的偵測不易。因此需要合適樣品前處理方法降低基質干擾或濃縮樣品，如果能夠兩者兼具是最好。 本研究利用電透析技術作為樣品前處理的方法，探討利用不同的膜材質、膜孔洞、電壓、通電時間、電解液離子濃度與電解液pH值等變化下，對於電透析之相對傳輸率的影響。在測試的分析物則選擇酚酸與食用色素兩種。 結果顯示電壓、電透析時間愈長，傳輸的效果愈好。但膜材質與孔洞大小對測試分析物傳輸影響不大。然而電解液的pH值對酚酸傳輸有明顯的改變，對色素分子則差異不明顯。 論文最後測試電透析方法在降低樣品中基質干擾的可行性，並証明藉由多次電透析處理樣品，是否能達到濃縮分析物的效果。
關鍵字
電透析並列摘要
Abstract Capillary electrophoresis(CE) that has advantages of high separation ability and high efficiency, is an important analytical technology now. But it is difficult to determine analyte contents if serious matrix interference exist in real sample, or analyte concentration is too low. Thus a good sample pretreatment method is necessary in order to decrease the matrix interference and enhance analyte concentration in food samples. In this study, an electrodialysis method was used as a sample pretreatment, The effect of membrane type, pore size of membrane, applied potential and time of electrodialysis, electrolyte concentration, and solution pH was examined, in order to achieve higher relative transport rate (R.T.R.) of phenolic acids and colorants. That were used as the test analytes in this study. The result displayed that the applied potential and time of electrodialysis affected obviously the R.T.R. for both phenolic acids and colorants, but their R.T.R.s were rarely altered by the type and pore size of membranes. On the other hand, a higher pH electrolyte solution could improve the R.T.R. of phenolic compounds, but had slightly influence on the R.T.R. of colorants. Finally, this study tested the ability of electrodialysis method in reducing sample matrix, and also examine the feasibility of repeated electrodialysis technology in enhancing phenolic acid and colorant concentration.