探討中藥萃取物接枝於聚酯不織布之生物活性

Evaluation of chinese herb extracts grafted onto poly(ethlene terephthalate) nonwoven fabric for bioactivity

蘇彥如(Yen-Ju Su)

指導教授 ： 翁清松

中原大學/工學院/醫學工程研究所/碩士(2007年)

https://doi.org/10.6840/cycu200700789

摘要

近年來，中草藥及天然植物藉由不同的萃取技術以及分離純化不同的有效成分，由於天然物中所含有之成分相對於化學藥劑所含有的副作用，天然植物及中草藥的研究與開發逐漸備受重視。目前皮膚的相關研究，大多應用於傷口癒合以及化妝品，以不破壞和傷害皮膚組織為原則，針對抗發炎性以及抗菌性的效果，為主要研究的基礎及目的。因此本實驗利用天然的中藥萃取物取代化學合成之藥劑，應用於皮膚以達到抗菌及抗發炎之效能，並作為中草藥抗菌性及抗發炎性相關應用之研究基礎。
本實驗利用文獻記載具有抗發炎性及抗菌性功效之中藥材：當歸、白芷、白朮、紫草及槐花，以水利用索氏萃取法進行中藥成分萃取，且對於中藥原藥材及萃取物分別進行抗菌性之試驗，並觀察中藥萃取物在不同濃度下 (0.1、1、10 mg/ml) ，對於纖維母細胞以及巨噬細胞之影響，以了解萃取物之細胞毒性及抑制一氧化氮生成之功效。且本研究運用射頻電漿對於聚酯不織布進行表面活化處理，以不同接枝方式 (Pad-Dry 及 Chemical-grafting) 接枝萃取物於材料表面，之後以掃描式電子顯微鏡及能量分散光譜儀進行材料結構之分析，並以接觸角觀察材料之親水化性質，最後對於材料進行生物活性之測試：抗菌性試驗及材料細胞毒性。
研究結果發現，中藥之萃取產率以當歸為最佳。就抗菌性分析中，原藥材僅以白芷及白朮對於表皮葡萄球菌有明顯的抑菌效果；而對於中藥萃取物之定量測試中，結果顯示以10 mg/ml的抑菌率為最佳，其中對於金黃色葡萄球菌以白朮萃取物抑菌率最佳，而以白芷萃取物對於表皮葡萄球菌及綠膿桿菌具有最佳效果。中藥萃取物對於細胞之影響，以紫草及槐花萃取物對於纖維母細胞有明顯增生的效果；而對於與發炎反應相關的巨噬細胞，以 0.1μg/ml 的脂多醣誘導巨噬細胞活化，研究發現添加紫草及槐花萃取物後細胞有增生的趨勢；而對於發炎反應所分泌出的一氧化氮，以紫草及槐花的萃取物及兩種之混合液均有明顯抑制一氧化氮生成量。最後以 4 種方式接枝萃取物於聚酯不織布製成中藥敷材，以 plasma-chemical grafting (P-CG) 的方式的接枝率為最佳，且接枝萃取物後均有明顯改善親水性。而由掃描式電子顯微鏡觀察敷材表面，推測中藥萃取物有接枝於纖維表面，且以P-CG的方式接枝量較多。對於敷材的生物活性，以 P-CG 方式接枝白朮對於金黃色葡萄球菌抑菌效果較佳，而對於表皮葡萄球菌及綠膿桿菌以 P-CG方式接枝白芷效果較佳。而敷材之細胞毒性結果顯示，大部分敷材之細胞毒性均為 0 級，具有良好的生物活性。因此，本研究發現以高濃度 (10mg/ml) 白芷萃取物具有最佳抑制細菌生長的作用，而其中以高濃度 (10mg/ml) 紫草萃取物具有良好之抗發炎性作用，未來可嘗試將此兩種中藥萃取物相互混合，以達到具有最佳抗菌性及抗發炎性之功效，進而加以促進傷口的癒合。

關鍵字

抗菌活性 ； 抗發炎性 ； 中藥萃取物 ； 聚酯不織布 ； 射頻電漿

並列摘要

The different extracted techniques and separation methods with Chinese herbal medicine refine many compounds which have anti-bacterial and anti-inflammation in recent years. The study and development of Chinese herbal medicine are popular because the property of Chinese herbal medicine is better than the toxicity of chemistry. The main purpose of the study was to evaluate to the effect of anti-bacterial and anti-inflammation of Chinese herbal extracts onto PET nonwoven fabric .
In this study, Five Chinese herbs - Angelicae Radix, Angelicae Dahuricae Radix, Atractylodis Rhixoma, Lithospermi Radix and Sophora japonica L. were extracted by Soxhlet extractive process. In antibacterial property study, three different concentrations (0.1, 1, 10 mg/ml) of the Chinese herbal extracts were against gram-positive bacteria (Staph. aureus and Staph. epidermidis) and gram-negative bacterium (Psendomonas aeruginosa). Additionally, because the number and change of fibroblast will affect the state of healing during the wound healing, the effects of Chinese herb extracts on wound inflammatory and healing situation were observed in the fibroblasts and macrophage culture assay. In the process of making dressing, poly(ethylene terephthalate) nonwoven fabric was pretreated using radio frequency plasma to increase surface activation, followed by Pay-Dry or chemical grafting with Chinese herb extracts. The dressings were characterized using Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometers (EDS), contact angle, antibacterial and cytotoxicity assasys.
The results showed the extract of Angelicae Radix. yielded more than others. In antibacterial assay, Atractylodis Rhixoma was the best antibacterial activity for Staph. aureus, and Angelicae Dahuricae Radix was the best antibacterial activity for Staph. epidermidis and Psendomonas aeruginosa, and the thickness of extracts was inhibited of bacterial increasingly. The cytotoxicity of the Lithospermi Radix and Sophora japonica L. extracts increased cell survival with fibroblast and macrophage by the MTT assay. In anti-inflammation assays, the effect of five extracts on Lipopolysaccharide (0.1μg/ml) induced production of nitric oxide (NO) , and all extracts inhibited the NO product, especially Lithospermi Radix and Sophora japonica L..
In the results of the herbal dressing test, Sophora japonica L. extracts with Plasma-Chemical Grafted (P-CG) method was the best by the grafted rate. As for the characteration of dressings, the results showed the dressings with grafted extract were more hydrophilic than untreated PET nonwoven. The assays on the bioactivity of the dressing, the dressing grafted Atractylodis Rhixoma with P-CG method inhibited the growth of Staph. aureus, and the dressing grafted Angelicae Dahuricae Radix with P-CG method inhibited the growth of Staph. epidermidis and Psendomonas aeruginosa. The results on the cytotoxicity of the dressings, the cytotoxicity of heral dressings almost were 0 grade to the test standard. It meant that the herbal dressings had no cytotoxicity. Therefore, the results showed that Angelicae Dahuricae Radix extract was the best antibacterial activity and Lithospermi Radix extract was the best anti-inflammation activity on the thickness concentration. For enhancing wound healing, we could try to mix them together (Angelicae Dahuricae Radix extract and Lithospermi Radix extract) in the future.

並列關鍵字

Chinese herbal extract ； Anti-inflammation ； Radio frequency plasma ； Poly(ethylene terephthalate) nonwoven fabric ； Anti-bacterial activity

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