膜過濾已廣泛應用於化工、食品及生化等程序中的膠體微粒或蛋白質等之濃縮分離,於其過濾操作過程中,膜結垢是導致其濾速大幅下降因素之一,而影響膜結垢之因素甚多,除了操作壓力與膜面之流力作用外,蛋白質與膜面之交互作用力亦常是重要之因素。本研究系以BSA與LY等蛋白質溶液進行膜材上之吸附與過濾實驗,探討於靜態吸附下,膜材吸附蛋白質後,其上接觸角角度改變程度,與蛋白質溶液吸附膜材後其上電性之變化,藉SEM觀察膜面吸附蛋白質情況;另外,以不同操作條件進行掃流超過濾實驗,配合XDLVO理論估算蛋白質與薄膜間之作用能量,探討其交互作用能量與其過濾行為之關聯,並與傳統DLVO理論比較,分析親疏水作用能量對過濾行為之重要性。
Membrane filtration has been widely used in chemical, food and biochemical processes such as for colloidal suspension separations. Membrane fouling is still a troublesome problem in the application of membrane filtration. Since the interactions between the colloids and membrane generally is the key factor in controlling the fouling in the initial stage of ultrafiltration, it is important to evaluate quantitatively the interactions between the colloidal target and the membrane for analyzing the fouling mechanism in ultrafiltration. The objective of this study was to investigate the effect of chemical interactions between protein and membrane and that between protein each other on the ultrafiltration performances. Experiments of adsorption and crossflow ultrafiltration were carried out with BSA and Lysozyme solutions. An extended DLVO(called XDLVO) model was used in the study to account for the additional interaction induced by the polarity of the solvent (acid-base interactions) into the total interactions and also compared to the interactions evaluated by the classical DLVO theory. The contact angle and the zeta potential on membrane surface after protein adsorption were measured to determine the parameters required for applying XDLVO model. In addition, crossflow ultrafiltration experiments were carried out and the filtration fluxes under different operation conditions were compared qualitatively with that predicted based on the interaction energy evaluated by the two different models.