- 學位論文
合成一維奈米金線為生物感測器電極之研究
Synthesis of 1D gold nanowire electrode for the Study of Biological Sensors
摘要
本論文成功地利用電化學沉積法在聚碳酸酯膜(PC膜) 製備金奈米線。使用3種方法來對奈米金電極進行修飾，增強其靈敏度。金奈米線電極會固定血紅素（Hb）因此，用血紅素修飾奈米金電極可以作為一個具有高靈敏度的血紅素偵測器。一維電極有較大的表面積比平板電極的電催化效果大很多。修飾過的奈米金電極以循環伏安儀進行檢測，此外簡單的在奈米金電極覆蓋一層葡萄糖氧化酶( GOD )，藉由循環伏安儀也可檢溶液中測葡萄糖含量。使用葡萄糖氧化酶(GOD) 修飾電極時，隨著葡萄糖溶液濃度比例的上昇，其氧化還原電流亦有隨之增加的趨勢，故此方式也可用在糖尿病的檢定方法。由實驗結果可知 Fe3O4 奈米粒子修飾過的電極，確實可以有效提升並增益其應答電流，進而增加感測器之靈敏度，主要原因是Fe3O4導電機制是Fe2+與Fe3+之間跳躍傳遞的關係，可以幫助赤血鹽的電子傳遞且Fe3O4等電點為7.4，在奈米微粒表面帶有正電，因此會與赤血鹽，進一步形成靜電吸附，造成電極表面濃度增加，進而具有促進電子傳遞速率。因為奈米金管的表面積大於奈米金線的表面積，所以奈米金管的應答電流大於奈米金線，這更加印證了奈米金電極因表面積的增大，可以有效提升其應答電流。
並列摘要
Gold nanowires were successfully fabricated by electrochemical deposition using polycarbonate membranes (PC) as the growing templates. The Au nanowire electrodes were modified by three methods which enhanced the response of sensibility in different ways. The nanowire electrode was employed to be the support matrix for the immobilization of hemoglobin (Hb).. Therefore, the Hb-modified Au nanowire electrode could be used as a biosensor with high sensitivity for Hb. The 3D electrode has a larger surface area than that of flat electrode and the sensitivity of electrode for hemoglobin (Hb) was enhanced. The electrode of gold nanowires modified by glucose oxidase (GOD) is suitable for application as a glucose sensor. The cyclic voltammorgrams (CV) demonstrated that the response current of glucose was enhanced by using GOD-modified Au nanowires working electrode. Enhanced cyclic voltammorgrams were also obtained using Fe3O4 nanoparticals modified gold nanowires as the working electrode. It is thought that the electron transfer between Fe2 + and Fe3 + ions helped the activity of ferricyanide.