- 學位論文
應用於脂締素檢測霍爾元件感測系統
Hall Sensor Based System Development for Adiponectin Detecting Application
摘要
此系統以檢測奈米級磁珠；在酵素連結免疫吸附分析法中含量的變化，藉以了解檢體中脂締素的含量。整個系統包含三大部分：一為前端脂締素檢測平台，由磁性奈米粒子取代傳統螢光標的物，利用其數量多寡的變化和目標物脂締素呈現正相關線性變化的關係，檢測檢體中脂締素含量的變化。二為傳統型霍爾元件感測陣列，因為分析法所使用到的感測面積大，故使用感測陣列進行分析檢測，以避免訊號在感測平台中分布不均勻。霍爾元件為一磁場檢測器，可以檢測磁珠在感測平台中增減含量對磁場的變化，並使用開關切換控制感測陣列的開啟關閉，和使用類比數位混和多工器切換輸出霍爾電壓。最後為霍爾電壓讀出訊號處理電路，對讀出的霍爾電壓進行位移和訊號放大處理，因為檢測對象微小；為6~10 nm的磁珠，使用放大電路提升靈敏度由40 V/μAT至1250 V/μAT，將背景雜訊除去使電路訊號統一，不受到背景雜訊干擾，使得讀出訊號具有客觀性和重複性。本論文使用TSMC035μm2P4M bioMEMS製程，在霍爾元件和讀出電路積體化後，使量測微米及奈米等級磁珠的元件靈敏度上升，加上後端霍爾電壓訊處理電路，使得訊號背景雜訊移除與放大倍率的調整，最後得到可靠的霍爾電壓輸出訊號，加上前端感測平台為一完整的檢測系統。
並列摘要
This system is used to detect nano-beads, in the enzyme-linked immuno assay to inspect adiponectin. The entire system is consists of three parts: the detection platform, traditional array of sensing Hall elements, and read out for the Hall voltage signal processing circuit. The detection platform is used for adiponectin. We use nano-particles magnetic beads to replace the traditional enzyme linked antibody. The magnetic beads and the adiponectic have linear relationship. The traditional array of sensing Hall elements is used in the sensing area. To avoid the uneven distribution of signal in the sensor platform, the sensor arrays is used for analysis and detection. Hall element is used to detect the changes in the magnetic bead, which causes the magnetic field to change. Switch is used to control the sensor array. The mixed analog multiplexer is used to determine digital output Hall voltage. The read out for the Hall voltage signal processing circuit is used for reading out the Hall voltage displacement amplification and signal processing. The detection of small objects of 6~10 nm of the beads must be used to enhance the sensitivity of the amplifier circuit 40 V / μAT up to 1250 V / μAT. With different testing environment, background noise is different; therefore it is reduced so that the circuit will have an accurate result. This paper uses TSMC 0.35μm 2P4M bioMEMS process. After the integration of Hall element and the readout integrated circuit technology, hall element sensitivity will be increased.