螢光金奈米團簇於細胞生物相容性的研究

近來，對於將金奈米粒子用在偵測追蹤癌症方面被廣泛研究，關於粒子之基本物理特性、細胞吸收粒子及預防腫瘤產生方面也有持續的研究發表。隨著科技進步，目前已可依照需求製作出不同特性的金奈米粒子，有研究報導指出，金奈米粒子可藉由特殊peptide運送，進入癌細胞核內，造成癌細胞DNA受損，進而使癌細胞死亡。本研究主要利用人類表皮癌細胞（Human Epidermal Carcinoma A431 Cells）與接枝硫辛酸的螢光金奈米團簇（Flurorecent gold nanoclusters, FANCs）共同培養，觀察FANC與A431 cells培養後，不同時間點的差異、FANC進入A431 cells後，行繼代實驗的情況、不同濃度的FANC對A431 cells形態之影響及進行FANC對細胞毒性分析，並偵測FANC與A431 cells培養後活性氧族群的變化。結果顯示，FANC並不會對細胞造成毒性傷害，且大部分的FANC分布於細胞質，並圍繞於細胞核周圍，而FANC隨著繼代次數的增加，螢光也隨之減弱。本研究同時也針對神經幹細胞（Neuronal Stem Cells, NSCs）進行分析，將FANC與NSC共同培養，發現隨著NSC的增生，FANC也有聚集現象產生，另外也進行細胞毒性分析。

關鍵字

螢光金奈米團簇；人類表皮癌細胞株；神經幹細胞； Lipofectamine

並列摘要

Recently, many research studies have been established to explore gold nanoparticles as cancer tracer. In addition, the studies of chemical and biological properties of gold nanoparticles regarding to the basic physical, cellular uptake and cancer prevention have been also published continuously since twentieth century. Using advanced technology, the different type of gold nanoparticles can be manufactured adjusted to your needs. Some research studies pointed that gold nanoparticles could be transported into nucleus by targeting specific peptide for causing DNA damage in cancer cell and making it undergo apoptosis. In the present study, the Human Epidermal Carcinoma A431 cell lines were cultured with various concentration of flurorecent gold nanoclusters （FANCs）which are grafted with lipoic acid （DHLA, dihydrolipoic acid）. Cellular uptake, cell-passage ability, cell morphology were observed using fluorescent microscopy. Cell viability was analyzed by MTT assay. The result showed that the cancer cells were not seriously damaged by the FANCs. FANCs were most distributed in cytoplasma. Some FANCs were present around the nucleus. The more number of cells have been passaged, the greater the fluorescence decreases. We also cultured neuronal stem sells with FANCs, and found that the more number of NSCs accumulate, the greater the FANCs aggregate

並列關鍵字

Lipofectamine ； Neuronal stem cells ； Fluorescent gold nanoclusters ； A431 cell lines

參考文獻

Bao, H., Jia, C.P., Zhou, Z.L., Jin, Q.H., and Zhao, J.L. (2009). A Novel DNA Detection Method Based on Gold Nanoparticle Probes and Gene Chips. Acta Chim Sinica 67, 2144-2148.

Boyd, G.T., Yu, Z.H., and Shen, Y.R. (1986). Photoinduced luminescence from the noble metals and its enhancement on roughened surfaces. Physical Review B 33, 7923-7936.

Cho, H.J., Yoon, H.Y., Koo, H., Ko, S.H., Shim, J.S., Lee, J.H., Kim, K., Kwon, I.C., and Kim, D.D. (2011). Self-assembled nanoparticles based on hyaluronic acid-ceramide (HA-CE) and Pluronic (R) for tumor-targeted delivery of docetaxel. Biomaterials 32, 7181-7190.

Dahl, J.A., Maddux, B.L.S., and Hutchison, J.E. (2007). Toward greener nanosynthesis. Chem Rev 107, 2228-2269.

Derfus, A.M., Chan, W.C.W., and Bhatia, S.N. (2004). Probing the cytotoxicity of semiconductor quantum dots. Nano Lett 4, 11-18.

國際替代計量

螢光金奈米團簇於細胞生物相容性的研究

全文下載

主題瀏覽