應用於免疫分析之微流道平台設計

MICROFLUIDIC PLATFORM FOR IMMUNOASSAY

尼克其(Nikhil Bhalla)

指導教授 ： 鍾文耀

中原大學/工學院/電子工程研究所/碩士(2012年)

https://doi.org/10.6840/cycu201200718

摘要

本研究完成了微流道酵素免疫分析平台的探討與設計。此論文包含了兩種設計，並討論製造和測試結果，其ㄧ以連續式微流道的設計方式完成;其二則是以離散式微流道實現。在連續式微流道平台中，以微米等級通道和微型儲存槽等技術並透過聚二甲基矽氧烷 (Poly-Di-Methyl-Siloxane) 實現在玻璃平台,上此設計使用存放
六種不同試劑的六個儲存槽、ㄧ個塗有硝酸纖維素薄膜(nitrocellulose membrane) 的
反應槽和ㄧ個廢液儲存槽。而在離散式微流道的設計中，不同地則是使用一組電極以電濕潤原理(electrowetting principle) 控制流體移動. 最後，本研究更是在磁珠疫分析法做了詳盡的探討與研究，透過模擬和數學分析證明超順磁性磁珠可完成酵素免疫分系法的功能無誤，本論文並提供了可和現有技術相互比較的學術參考。

關鍵字

微流道 ； 免疫

並列摘要

The work accomplished in this thesis discusses the study and design of microfluidics based immunoassay platform. Two designs, one based on continuous flow of fluid and other on discrete flow of liquid has been discussed. It includes the design, fabrication and testing results of both the platforms. The approach based on continuous flow of fluid is where the platform involves micro-channel and micro-chambers built with Poly-Di-Methyl-Siloxane (PDMS) on glass. The design involves six reagent storage chambers, one reaction chamber and one waste disposable chamber. The chamber where the reaction takes place was coated with nitrocellulose membrane. The second design uses a set of electrodes using electrowetting principle to move the fluid over them. Detailed study on magnetic particle immunoassay has been done. Simulations and mathematical analysis done shows the capability of carrying immunoassay using super-paramagnetic particle. In addition it gives an insight of previous work and compares the available techniques in microfluidic technology in literature.

並列關鍵字

microfluidics ； immunoassay

參考文獻

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