大麒麟菜多醣製備及水解後抗氧化性測定之研究

Analysis of the Antioxidant activity of Eucheuma cottonii Polysaccharide and Oligosaccharide

黃士銓(Shih-Chuan Huang)

指導教授 ： 鍾財王

中原大學/工學院/化學工程研究所/碩士(2019年)

https://doi.org/10.6840/cycu201900377

摘要

早期的人們把海藻作為糧食的供應源之一，但隨著時代的進步，海藻被研究出更具有利用價值的特性，如抗菌抗腫瘤、抗氧化、保濕功能等。海藻來源廣泛，成本低廉，本身含水量高，具有良好的抗菌抗氧化性，是人們添加至護膚品中用來美容護膚的好選擇。
本研究將以大麒麟菜生產多醣為主要目標，進一步探討水解及其抗氧化性。探討在不同操作參數下造成目標結果的差異。研究中，利用回應曲面法針對固液比(1：60, 1：70, 1：80)、萃取溫度(75, 80, 85℃)以及水解時間(3,4,5 hr)進行最佳化探討，並獲得最大多醣產率18.17 mg/ml、多酚產率8.567 μg/mL、DPPH自由基清除能力為20.85 %、還原力吸光值為0.086 Abs、亞鐵離子螯合能力為90.37%。
接著探討酵素水解部分，多酚產率由9.23提升至11.98 μg/mL，DPPH自由基清除能力由24.20提升至38.12 %，還原力吸光值由0.0845提升至0.1265 Abs，亞鐵離子螯合能力由90.37提升至92.42 %。
比較大麒麟菜萃取液水解前後抗氧化性之差異試驗中，可明顯看出水解後的抗氧化性有明顯的提升。綜合上述大麒麟菜寡糖混合物具有開發做為抗氧化功能之化妝品原料深具潛力。

關鍵字

大麒麟菜多醣 ； 多醣萃取 ； 回應曲面法 ； 酵素水解 ； 小分子寡糖

並列摘要

In this study, the Freeze-dried Eucheuma cottonii was used as raw material and extracted the polysaccharide with 0.1 M sodium hydroxide aqueous solution. Cellulase were used to hydrolyze the polysaccharide and the results showed that most Eucheuma cottonii polysaccharide after enzymatic hydrolysis system were hydrolyzed to smaller molecular oligosaccharide.
The results from Response surface methodology showed that the maximum responses as following：polysaccharide concentration was 18.17 mg/mL, polyphenol was 8.57 ug/mL, DPPH radical scavenging activity was 20.85%, absorbance of reducing power was 0.086, ferrous ion chelating ability was 90.38%.
After enzymatic hydrolysis system, polyphenol was upgrade to 11.98 ug/mL, DPPH radical scavenging activity was upgrade to 38.12%, absorbance of reducing power was upgrade to 0.1265, ferrous ion chelating ability was upgrade to 92.42%.
Comparing the differences in the antioxidant activity with different procedures, we found that enzymatic hydrolysis helps to increase the antioxidant activity of Eucheuma cottonii. That means it has great potential to develop cosmetic raw materials for antioxidant function.

並列關鍵字

Eucheuma cottonii ； Polysaccharide extraction ； Response surface methodology (RSM) ； hydrolysis process ； oligosaccharide

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