以碳化金屬有機凝膠結合高分子整體成形管柱固相微萃取兒茶酚胺類化合物

本研究首次以金屬有機凝膠製備之孔洞性碳材(porous carbon derived from metal-organic gels)為碳化金屬有機凝膠(carbonized metal-organic gels, cMOGs) cAl-BDC-NH2結合整體成形高分子製備萃取管柱(cAl-BDC-NH2-polymer monolithic column)，對兒茶酚胺類化合物(catecholamines)進行固相微萃取(solid-phase microextraction分, SPME)。實驗過程中首先以cAl-BDC-NH2混摻於聚甲基丙烯酸酯類整體成形高子(cAl-BDC-NH2-poly(BMA-EDMA))進行萃取參數最佳化，並微胞電層析(micellar electrokinetic capillary chromatography, MEKC)檢測各萃取步驟結果，最後比較金屬有機凝膠未碳化(Al-BDC-NH2-poly(BMA-EDMA))、不同配位官能基(cAl-BDC-poly(BMA-EDMA))、不同金屬中心(cCr-BDC-NH2-poly(BMA-EDMA))及混摻不同整體成形高分子(cAl-BDC-NH2-poly(Sty-DVB))對兒茶酚胺萃取效果差異。結果顯示最佳條件為36% 含量之cAl-BDC-NH2-poly(BMA-EDMA)、5 cm管柱長、pH 7之5 mM磷酸鹽樣品基質、ACN為清洗溶劑及pH 2之40 mM磷酸鹽緩衝液含30%甲醇為脫附溶劑，最佳萃取回收率為50.3%至97.2%。本研究成功製備cMOGs-polymer萃取管柱，並應用於SPME對高極性生物性分析物兒茶酚胺類化合物進行萃取。

關鍵字

固相微萃取；兒茶酚胺；孔洞性碳材；金屬有機凝膠

並列摘要

In the present study, the porous carbon material derived from metal-organic gels (cMOGs) was defined as cAl-BDC-NH2, which embedded with polymer monolithic column for solid-phase microextraction (SPME) of catecholamine. In this experiment, cAl-BDC-NH2-poly(butyl methacrylate-ethylene dimethacrylate) (cAl-BDC-NH2-poly(BMA-EDMA)) was used to optimize the extraction parameters, and the results of the extraction steps were detected by micellar electrokinetic capillary chromatography (MEKC). Finally, the compare of the catecholamine extraction effect with metal-organic gels (Al-BDC-NH2-poly(BMA-EDMA)), different metal centers (cCr-BDC-NH2-poly(BMA-EDMA)) and embedded with different integrally formed polymers (cAl-BDC-NH2-poly(Sty-DVB)). The results showed that the optimum conditions were 36% cAl-BDC-NH2-poly(BMA-EDMA), 5 cm column length, 5 mM phosphate sample matrix at pH 7, ACN as washing solvent and 40 mM phosphate buffer in 30% methanol at pH 2 as the desorption solvent, the recovery was 50.3% to 97.2%. In this study, cMOGs-polymer extraction column were successfully prepared and applied to SPME of catecholamines compounds, which were highly polar biological analytes.

並列關鍵字

solid-phase microextraction ； catecholamines ； porous carbon materials ； metal-organic gels

參考文獻

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以碳化金屬有機凝膠結合高分子整體成形管柱固相微萃取兒茶酚胺類化合物

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