酸桔葉部精油成分之研究

中文摘要酸桔，學名Citrus sunki Hort. Ex Tanaka，英名Sunki，為芸香科(Rutaceae)植物。採取酸桔葉子，經清洗、陰乾、打碎後，利用間接式水蒸汽蒸餾法(Indirect steam distillation)抽取酸桔葉中精油成份。經溶劑萃取、鹼處理、水洗與濃縮等步驟，取得淡黃褐色的中性精油。酸桔葉部之精油平均收油率為0.60%。酸桔之中性精油經管柱色層分析粗分，分成碳氫部和含氧部化合物兩大部分，而碳氫化合物佔中性精油51%，含氧化合物佔中性精油49%。酸桔之中性精油經管柱色層分析細分，使用氣相層析儀(GC)、氣相層析質譜儀(GC/MS)、傅立葉轉換紅光譜儀(FTIR)、核磁共振光譜儀(NMR)等儀器分析鑑定其化學成分，共確認了二十八個成分；碳氫部化合物有α-Pinene、β-Pinene、D-Limonene、Z-Ocimene、p -Cymene 、γ-Terpinene、Terpiolene、β-Elemene、Caryophyllene、Cadinene、α-Bergamotene、α-Caryophyllene、α-Farnesene、α-Selinene、Eremophilene、(Z,E)-α-Farnesene、 Copaene等十七個成分，含氧部化合物確認了Linalool oxide、β-Linalool、p-Cymen-8-ol、Methyl thymyl ether、trans-Nerolidol、Spathulenol、α-Cadinol 、Caryophyllene oxide、Hexahydrofarnesyl acetone、Isophytol、Phytol等十一個成分。

關鍵字

酸桔

並列摘要

Abstract Citrus sunki Hort. Ex Tanaka, name Sunki, is one type of Rutaceae. In this research, leaves of sunki were collected, washed, dried and fragmented. The neutral essential oil was obtained through indirect steam distillation, and the steps of solvent extraction, base treatment, dehydration and condensation. The average oil extraction rate of sunki leaves is 0.6%. The neutral essential oil was isolated and purified by chromatography, dividing into hydrocarbon and hydroxy compounds, with the former taking 51% of the content and the latter 49%. Twenty-eight compounds were identified through comparison among the spectra of GC, GC/MS, IR and NMR. The hydrocarbon compounds include α-Pinene, β-Pinene, D-Limonene, Z-Ocimene, p–Cymene, γ-Terpinene, Terpiolene, β-Elemene, Caryophyllene, Cadinene, α-Bergamotene, α-Caryophyllene, (Z,E)-α-Farnesene, α-Farnesene, Copaene, α-Selinene, Eremophilene1 17 compounds. Hydroxy compounds contain Linalool oxide, β-Linalool, α-Cadinol, p-Cymen-8-ol, Methyl thymyl ether, trans-Nerolidol, Spathulenol, Caryophyllene oxide, Hexahydrofarnesyl acetone, Isophytol and Phytol 11 compounds.