臺灣香檬萃取物對小鼠非酒精性脂肪肝之保護效果的統計研究

在現今這個資訊發達的世代，我們的生活範圍越來越為廣泛，長時間下來也產生了許多對於身體不好的習慣，像是熬夜通宵，導致日夜顛倒，或者是交際應酬…等等，讓我們的肝臟功能出現了問題。在臺灣這個有著水果王國之稱的地方，產出了臺灣香檬，也有「扁實檸檬」的稱號，是一種柑橘類水果，不管是果皮，還是果肉都含有豐富營養成分，在果皮之中屬川陳皮素(Nobiletin)及橘紅素(Tangeretin)的含量最高，目前有研究證實此水果具有助於抗癌、抗發炎、降三高(高血壓、高血脂、高血糖)等等功效。本研究探討關於臺灣香檬果皮萃取物在高脂飲食所誘導的小鼠非酒精性脂肪肝的保護成效，此實驗為期18週，總共分為六組，分別為控制組(Control)、高脂飲食組(HFD)、高脂飲食配合臺灣香檬果皮萃取的低劑量組(HFD + CDEEL_L)、高脂飲食配合臺灣香檬果皮萃取的高劑量組(HFD + CDEEL_H)、高脂飲食配合臺灣香檬果皮萃取物之川陳皮素組(HFD + Nob)以及高脂飲食配合臺灣香檬果皮萃取物之橘紅素組(HFD + Tan)，對以上六組進行比對。在第18週犧牲後，對小鼠進行心臟血液測量，分別有丙胺酸轉胺酶(Alanine aminoTrans-ferase, ALT)、天門冬胺酸轉胺酶(ASpartate aminoTransferase, AST)、三酸甘油脂(Triglyceride, TG)、總膽固醇(totalcholesterol, TC)、體脂率、副睪脂肪以及腎週脂肪，根據以上數據進行統計分析。此實驗是使用R及SPSS統計軟體進行資料分析，以變異數分析(one-way ANOVA)及無母數統計為主要分析方法。

關鍵字

臺灣香檬萃取物；變異數分析；無母數統計

並列摘要

In today's information-developed era, the scope of our life is becoming more and more extensive, and many habits that are not good for health have also developed over a long period of time, such as staying up all night, causing day and night to be reversed, or socializing...etc., and then liver problems comes out. Taiwan which has the title of the fruit kingdom, has produced Citrus depressa Hayata, also known as flat lemon, it is a citrus fruit, both the peel and the pulp are rich in nutrients, among the peels are Nobiletin and Tangeretin. It has the highest content, and current studies had confirmed that this fruit was helpful for anti-cancer, anti-inflammatory, lowering blood pressure, lowering high blood lipids, lowering high cholesterol... and so on. This study investigated the protective effect of Citrus depressa Hayata peel extract on non-alcoholic fatty liver disease in mice induced by high-fat diet. The experiment lasted for 18 weeks and was divided into six groups. They were the control group (Control), the high-fat diet group (HFD),the high-fat diet combined with the low-dose group of Citrus depressa Hayata peel extract (HFD + CDEEL_L), the high-fat diet combined with the high-dose group of Citrus depressa Hayata peel extract (HFD + CDEEL_H), high-fat diet combined with Nobiletin of Citrus depressa Hayata peel extract (HFD + Nob), and high-fat diet combined with Tangeretin of Citrus depressa Hayata peel extract (HFD + Tan), and then compared the above six groups. After sacrificing at week 18, the mice were measured to cardiac blood measurements, including ALanine aminoTransferase (ALT), ASpartate aminoTransferase (AST), Triglyceride (TG), totalcholesterol (TC), body fat ratio, epididymal fat and perirenal fat, statistical analysis was based on the above data. In this experiment, R and SPSS statistical software were used for data analysis, and one-way ANOVA and nonparametric statistics were used as the main analysis methods.