近年來免疫醫學的發展使得許多疾病得以獲得有效治療,其中使用免疫細胞進行功能強化並用於癌症治療的發展,已成為重要的科學研究領域 。本研究擬設計一熱響應型共聚網絡狀界面來控制人體血液中免疫細胞的捕捉功能。在實驗系統的設計,採用控制不同混合單體的比例來調整網絡結構界面的溫度響應性質的變化。在材料檢測方面,使用傅立葉轉換紅外光譜儀 (Fourier transform infrared spectrometer, FTIR) 與接觸角量測儀所製備水凝膠材料的基本物化性質。透過酵素免疫吸附分析法 (Enzyme-linked immunosorbent assay, ELISA) 可以量化測定蛋白質於水凝膠網絡結構界面的吸附量。本研究以雷射共軛聚焦顯微鏡 (Confocal laser scanning electron microscope, CLSM) 觀察免疫細胞於樣品表面的吸附量,最後使用流式細胞儀 (Flow cytometry) 探討免疫細胞的存活率。
In recent years, the development of immuno-medicine has enabled the effective treatment of fetal diseases. The use of immune cells for functional enhancement and development of cancer treatment has become an important scientific research field. In this study, a thermo responsive copolymer network interface was designed to control the capture function of immune cells in human blood. In the design of the experimental system, controlling the ratio of different mixed monomers was adopted to adjust the temperature-responsive properties of the interface of the network structure. In terms of material detection, the basic physicochemical properties of hydrogel materials prepared using Fourier transform infrared spectrometer and contact angle measuring instrument. The amount of protein adsorbed at the interface of the hydrogel network structure can be quantitatively determined by enzyme immunosorbent assay. In this study, the adsorption amount of immune cells on the surface of the sample was observed by a confocal laser scanning microscope, and finally, the survival level of immune cells was investigated by flow cytometry.