目錄 摘要 I Abstract II 致謝 III 目錄 IV 圖目錄 V 符號表 VII 第一章 緒論 1 1.1 發光二極體簡介 1 1.2 水殺菌簡介 2 1.3 文獻探討 3 1.4 研究目的 6 第二章 理論基礎、研究方法 7 2.1 照度計量測系統原理 7 2.2 Tracepro輻照度模擬 8 2.3 紫外線滅活實驗 10 2.3.1 紫外線殺菌原理 10 2.3.2 菌液製作 10 2.3.3 分光光度計測量菌液濃度 11 2.3.4 紫外線殺菌實驗 14 2.4 紫外線滅活模擬 15 第三章 結果與討論 17 3.1 輻照度量測 17 3.2 輻照度模擬 20 3.3 紫外線滅活實驗、模擬 30 第四章 結論與未來展望 41 4.1 結論 41 4.2 未來展望 42 參考文獻 43
The present study investigates inactivation effect of Escherichia coli K12 IFO 3301 by using homemade UVC LED module. UVC LED module is made by plural chips. The variations of average irradiation and irradiation distribution at different LED chips spacing distance and distance between sample light source are analyzed. Average irradiation and irradiation distribution are calculated by TracePro simulation and UVC light meter experiment. Residual Escherichia coli number after UV exposure is gained by spread plates. Effect of light source design on E. coli inactivation is analyzed in this study. The inactivation test of this study explores four different LED chips spacing distance of 5mm, 15mm, 25mm, 35mm and eight different distance between sample light source,15mm, 20mm, 25mm, 30mm, 35mm, 45mm, 55mm, 65mm. After inactivation test, average irradiation decreases because incident light reduces, when distance between sample and light source increases. Irradiation Uniformity of sample surface decreases because light sources become parallel light. After 60s UVC test, log inactivation first increases then decreases when distance between sample light source increases under same operating current and LED chips spacing distance. Log inactivation increases when LED chips spacing distance increases and is lower than 35mm under same operating current and distance between sample light source. Log inactivation decreases when LED chips spacing distance increases and is higher than 35mm. Because incident light becomes parallel light, and LED chips spacing distance increasing causes incident light and log inactivation reducing. Average irradiation and irradiation uniformity should be taken into account when designing disinfection reactor.