Total laboratory automation (TLA) proposes to improve the quality and efficiency of laboratory operations, and may provide a solution to the quality demands and staff shortages faced by today’s laboratories, systems occasionally become congested, particularly when laboratories face peak demand. This study integrates the value stream mapping, pull mechanism and parallel scheduling mode to improve the cycle time. An example of TLA of medical center to verify and validate this study, the cycle time can be improved from 2,987 s to 1,992 s, which is a 50% saving, the waiting time can be reduced from 1,474 s to 680 s, which corresponds to a total improvement of 216%.